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| 海马β2肾上腺素能受体激活对脓毒症相关性脑病焦虑样行为的影响 |
| Effect of activation ofhippocampal β2-adrenergic receptor on anxiety-like behavior induced by sepsis associated encephalopathy |
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| DOI：10.12089/jca.2019.05.017 |
| 中文关键词: 脓毒症 焦虑样行为 β2肾上腺素能受体 炎症 |
| 英文关键词: Sepsis Anxiety-like behavior β2-adrenergic receptor Inflammation |
| 基金项目:国家自然科学基金（81571083，81471105） |
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| 中文摘要: |
目的 探讨海马β2肾上腺素能受体激活对脓毒症相关性脑病焦虑样行为的影响。
方法 成年雄性C57BL/6小鼠76只，随机分为四组：sham+生理盐水组（A组，n=13）、sham+克伦特罗组（B组，n=13）、盲肠结扎穿孔（CLP）+生理盐水组（C组，n=25）、CLP+克伦特罗组（D组，n=25）。所有小鼠在术后即刻至术后15 d腹腔注射克伦特罗0.5 mg/kg或等容量的生理盐水。每组各取3只小鼠于术后第12 h处死取海马组织，检测小鼠海马内CD11b、IL-1β、IL-6及BDNF蛋白含量。每组剩余小鼠于术后第13、14天分别行旷场、高架十字迷宫实验。
结果 与A、B组比较，C组进入开放臂的时间缩短，次数减少；海马CD11b、IL-1β、IL-6蛋白含量明显增加，BDNF含量明显减少(P<0.05)；与C组比较，D组小鼠进入开放臂的时间明显延长、次数明显增加；海马CD11b、IL-1β、IL-6蛋白含量明显减少，BDNF含量明显增加（P < 0.05）。
结论 激活β2肾上腺素能受体可以改善脓毒症相关性脑病小鼠的焦虑样行为，这可能与其抑制小胶质细胞的激活，减少炎性因子的释放，增加BDNF的含量有关。 |
| 英文摘要: |
Ojective To observe the effects of β2-adrenergic receptor activation on the anxiety-like behavior induced by sepsis associated encephalopathy.
Methods Seventy-six adult male mice were divided randomly into four groups: sham operation with saline group (group A, n=13), sham operation with clenbuterol group (group B, n=13), cecal ligation and puncture with saline (group C, n=25), and cecal ligation and puncture with clenbuterol group (group D, n=25). Animals were subjected to an administration of clenbuterol 0.5 mg/kg or isochoric saline via intraperitoneal injection once daily ntil 15 day after surgery. Some mice in each group were sacrificed at 12 h post-surgery and the protein levels of microglial marker CD11b, inflammatory factors IL-1β, IL-6 and neurotrophic factor BDNF were detected by Western blot. The other mice were subjected to the open field test and elevated maze test on the day 13 and day 14 after surgery.
Results Compared with groups A and B, the time spent in the open arms and the number of entries into open arms were significantly decreased, the protein levels of CD11b, IL-1β, IL-6 were increased, and BDNF was reduced in group C (P < 0.05). Compared with group C, the time spent in the open arms and the number of entries into open arms were significantly increased, the protein levels of CD11b, IL-1β, IL-6 were decreased, and BDNF was increased in the group D(P<0.05).
Conclusion The activation of hippocampal β2-adrenergic receptor can relieve the anxiety-like behavior induced by SAE, which is related to its ability to reverse the neuroinflammation and promote the production of neurotrophic factors. |
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