文章摘要
右美托咪定对胃腺癌SGC-7901细胞生长和转移的影响
Effect of dexmedetomidine on proliferation, migration and invasion of human gastric cancer SGC-7901 cells
  
DOI：
中文关键词: 右美托咪定  胃癌  增殖  迁移  侵袭
英文关键词: Dexmedetomidine  Gastric cancer  Proliferation  Migration  Invasion
基金项目:
作者单位E-mail
童建华 210009,南京市,东南大学附属中大医院麻醉科  
姬宁宁 徐州医科大学江苏省麻醉学重点实验室 徐州医科大学附属医院麻醉科  
段满林 解放军南京总医院麻醉科  
周志强 解放军南京总医院麻醉科  
夏明 徐州医科大学江苏省麻醉学重点实验室 徐州医科大学附属医院麻醉科 xiaming1980@xzhmu.edu.cn 
徐建国 解放军南京总医院麻醉科  
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中文摘要:
      
目的：观察不同浓度右美托咪定对胃腺癌SGC-7901细胞增殖、迁移和侵袭的影响。
方法：人胃腺癌SGC-7901细胞接种于培养板培养24 h, 随机分为五组: 对照组(C组)、右美托咪定312.5 μg/ml组(D1组)、右美托咪定625 μg/ml组(D2组)、右美托咪定1 250 μg/ml组(D3组)和右美托咪定2 500 μg/ml组(D4组)。分别用不同浓度右美托咪定处理胃癌SGC-7901细胞48 h后, 采用CCK-8法、Transwell法检测细胞增殖、侵袭和迁移。
结果：与C组比较, D1、D2、D3和D4组SGC-7901细胞活力差异无统计学意义。D1、D2、D3和D4组SGC-7901细胞迁移能力明显高于C组(P<0.05或P<0.01)，且呈剂量依赖性；D1、D2、D3和D4组SGC-7901细胞侵袭能力明显高于C组（P<0.05或P<0.01），且呈剂量依赖性。
结论：右美托咪定可促进人胃癌细胞SGC-7901侵袭和迁移, 对其增殖无明显影响。
英文摘要:
      
Objective: To observe different behavior of proliferation, migration and invasion of SGC-7901 cells when exposured to dexmedetomidine of different concentrations.
Methods: Human gastric cancer cells SGC-7901 were inoculated on culture plate for 24 h, then were randomly divided into 5 groups: control group (group C), dexmedetomidine 312.5 μg/ml group (group D1), dexmedetomidine 625 μg/ml group (group D2), dexmedetomidine 1 250 μg/ml group (group D3), dexmedetomidine 2 500 μg/ml group (group D4). Each group was medicated and incubated for 48 h, then the cell proliferation, migration and invasion immediately were detected by CCK-8 and Transwell.
Results: SGC-7901 cell viability of groups D1, D2, D3和D4 had no significant difference compared with that of group C. The invasion ability and migration ability of SGC-7901 cells in groups D1, D2, D3 and D4 were significantly higher than those in group C (P<0.05 or P<0.01).
Conclusion: Dexmedetomidine can promote migration and invasion of SGC-7901 cells.
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