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| 半乳糖凝集素-1预处理对机械通气相关性肺损伤小鼠的影响 |
| Effect of galectin-1 preconditioning on ventilator-induced lung injury mice |
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| DOI：10.12089/jca.2023.11.013 |
| 中文关键词: 机械通气相关性肺损伤 半乳糖凝集素-1 细胞焦亡 小鼠 |
| 英文关键词: Ventilator-induced lung injury Galectin-1 Pyroptosis Mice |
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| 中文摘要: |
目的 探讨半乳糖凝集素-1（Galectin-1）预处理对机械通气相关性肺损伤（VILI）小鼠的影响。
方法 选择清洁级健康雄性C57BL/6小鼠30只，6～8周龄，体重22～30 g。采用随机数字表法将小鼠分为三组：对照组（C组）、VILI组（V组）和Galectin-1+VILI组（G组），每组10只。C组气管插管后保持自主呼吸4 h，V组和G组气管插管后机械通气4 h。气管插管前1 h C组和V组腹腔注射生理盐水0.75 ml，G组腹腔注射Galectin-1 3 μg。于气管插管前即刻、自主呼吸或机械通气结束时采集动脉血检测PaO2，后处死小鼠，收集肺泡支气管灌洗液（BALF），采用ELISA法检测BALF中IL-1β和IL-18浓度。取肺组织测定湿/干重比（W/D），采用qRT-PCR法检测肺组织GSDMD、caspase-1和caspase-11 mRNA表达量，Western blot法检测肺组织GSDMD、caspase-1和caspase-11蛋白含量，HE染色法观察病理改变并行肺损伤评分。
结果 与C组比较，V组和G组机械通气结束时PaO2明显降低，W/D、BALF中IL-1β和IL-18浓度、肺组织GSDMD、caspase-1、caspase-11 mRNA表达量及蛋白含量、肺损伤评分明显升高（P<0.05）。与V组比较，G组机械通气结束时PaO2明显升高，W/D、BALF中IL-1β和IL-18浓度、肺组织GSDMD、caspase-1、caspase-11 mRNA表达量及蛋白含量、肺损伤评分明显降低（P<0.05）。
结论 Galectin-1能够升高小鼠PaO2，降低IL-1β和IL-18浓度、经典及非经典细胞焦亡途径相关基因mRNA表达量和蛋白含量，减轻小鼠VILI。 |
| 英文摘要: |
Objective To investigate the effect of galectin-1 preconditioning on pyroptosis of ventilator-induced lung injury (VILI) in mice.
Methods Thirty clean grade healthy male C57BL/6 mice, aged 6-8 weeks, weighing 22-30 g, were divided into three groups by random number table method: control group (group C), VILI group (group V), and galectin-1 + VILI group (group G), 10 mice in each group. After endotracheal intubation, group C kept spontaneous breathing for 4 hours, groups V and G kept mechanical ventilation for 4 hours. One hour before endotracheal intubation, groups C and V were intraperitoneally injected with normal saline 0.75 ml, and group G was intraperitoneally injected with galectin-1 3 μg. Arterial blood was collected before endotracheal intubation and after spontaneous respiration or ventilation to detect PaO2. Then mice were sacrificed and bronchoalveolar lavage fluid (BALF) was collected. Concentrations of IL-1β and IL-18 in BALF were detected by ELISA. Lung tissue was collected for determination of the wet weight/dry weight ratio (W/D). The expression of GSDMD, caspase-1, and caspase-11 mRNA and protein in lung tissues were detected by qRT-PCR and Western blot. Pathological changes of the lungs were observed and scored by HE staining.
Results Compared with group C, PaO2 were significantly decreased, W/D, concentrations of IL-1β and IL-18 in BALF, mRNA and protein expressions of GSDMD, caspase-1 and caspase-11,and lung injury score were significantly increased in groups V and G (P < 0.05). Compared with group V, PaO2 was significantly increased, W/D, concentrations of IL-1β and IL-18 in BALF, mRNA and protein expressions of GSDMD, caspase-1, and caspase-11, and lung injury score were significantly decreased in group G (P < 0.05).
Conclusion Galectin-1 can increase PaO2 in mice and reduce IL-1β and IL-18 concentration, mRNA expression and protein content of classical non-classical pyroptosis pathway related genes, and reduce VILI in mice. |
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