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| 右美托咪定对睡眠剥夺小鼠记忆能力和突触可塑性的影响 |
| Effects of dexmedetomidine on memory fuction and synaptic plasticity in sleep-deprived mice |
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| DOI：10.12089/jca.2023.05.014 |
| 中文关键词: 右美托咪定 睡眠剥夺 突触可塑性 记忆 |
| 英文关键词: Dexmedetomidine Sleep deprivation Synaptic plasticity Memory |
| 基金项目:青年科学基金（8190051048） |
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| 中文摘要: |
目的 观察右美托咪定对睡眠剥夺小鼠记忆能力和突触可塑性的影响。
方法 选取SPF级雄性健康C57BL/6小鼠63只，6～8周龄，体重21～25 g。随机分为四组：对照组（C组，n=12）、右美托咪定注射组（D组，n=14）、睡眠剥夺组（S组，n=18）和睡眠剥夺+右美托咪定注射组（SD组，n=19）。S组和SD组小鼠给予持续3 d的睡眠剥夺，C组和D组小鼠相同时间内置入条件完全相同的剥夺仪中不进行睡眠剥夺。剥夺期间，D组和SD组小鼠24 h内尾静脉注射右美托咪定（每次100 μg/kg，每天13:00和15:00共两次），连续3 d；C组和S组小鼠同一时间尾静脉注射等容量生理盐水。剥夺结束后，实验第4天进行Y迷宫实验，实验第5天进行新物体实验，新物体实验结束后立即取海马组织，采用Western blot检测海马组织突触后PSD95蛋白含量。
结果 与S组比较，C组和D组摄食量明显增加（P＜0.05）；S组和SD组摄食量差异无统计学意义。与S组比较，C组、D组和SD组探索新物体时间比例及Y迷宫实验中进入正确臂循环次数所占比例明显升高，海马组织突触后PSD95蛋白含量明显升高（P＜0.05）。与C组比较，S组小鼠突触结构明显受损；与S组比较，SD组突触结构明显改善。
结论 右美托咪定可以改善睡眠剥夺小鼠记忆能力，促进突触可塑性。 |
| 英文摘要: |
Objective To observe the effects of dexmedetomidine on synaptic plasticity and memory function in the sleep-deprived mice.
Methods Sixty-three SPF healthy male C57BL/6 mice, aged 6-8 weeks, weighing 21-25 g, were randomly divided into four groups: control group (group C, n = 12), dexmedetomine injection group (group D, n = 14), sleep deprivation group (group S, n = 18), and sleep deprivation + dexmedetomine injection group (group SD, n = 19). Mice in groups S and SD were deprived of sleep for 3 days. The other two groups were only placed in a box without sleep deprivation. During sleep deprivation period, mice in groups D and SD were injected with dexmedetomidine (100 μg/kg each time, twice a day at 13:00 and 15:00) through a tail vein within 24 hours for 3 days, meanwhile, mice in groups C and S were injected with equal volumes of saline at the same time. The Y-maze test and the novel object recognition tests were performed on the 4th and 5th experiment's day, respectively. The hippocampal tissues of each group were collected immediately to determine postsynaptic PSD95 protein expression by Western blot after the behavioral experiments.
Results Compared with group S, the food intake in groups C and D were significantly increased (P < 0.05). There was no significant difference on the food intake in groups S and SD. Compared with group S, the proportion of time of exploring new objects and entering the correct arm cycle in the Y-maze test were significantly increased in groups C, D, and SD (P < 0.05), and the postsynaptic PSD95 protein content in hippocampal tissue was significantly increased (P < 0.05). Compared with group C, the synaptic structure in group S was significantly damaged. Compared with group S, the synaptic structure in group SD was significantly improved.
Conclusion Dexmedetomidine ameliorates memory dysfunction and improves synaptic plasticity in sleep-deprived mice. |
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