Objective To investigate the effects of penehyclidine hydrochloride postconditioning on cardiac function in myocardial ischemia-reperfusion (IR) rats.
Methods Thirty-two SPF healthy male Wistar rats, aged 8 weeks, weighing 220-250 g, were randomly divided into four groups: sham operation group (group S), sham operation combined with penehyclidine hydrochloride group (group SP), IR group (group IR), and IR combined with penehyclidine hydrochloride postconditioning group (group IP), 8 rats in each group. In group S, the left anterior descending coronary artery was only threaded without ligation, and normal saline 1 mg/kg was injected into the caudal vein 30 minutes later. In group SP, the left anterior descending coronary artery was only threaded without ligation, and penehyclidine hydrochloride 1 mg/kg was injected into the caudal vein 30 minutes later. In group IR, the left anterior descending coronary artery was ligated for 30 minutes, the ligation thread was loosened and reperfusion for 3 hours, and normal saline 1 mg/kg was injected into the caudal vein immediately before reperfusion (the ligation thread was loosened). In group IP, penehyclidine hydrochloride 1 mg/kg was injected into caudal vein immediately before release and reperfusion. Left ventricular end-diastolic pressure (LVEDP), left ventricular end-systolic pressure (LVESP), left ventricular ejection fraction (LVEF), and left ventricular shortening fraction (LVFS) were measured by transthoracic echocardiography, MAP of caudal artery was monitored with smart sphygmomanometer, the concentration of serum troponin T (cTnT) was detected by ELISA 5 minutes before chest opening, 3 hours after reperfusion and 6 hours after reperfusion.
Results Compared with 5 minutes before chest opening, LVEDP in groups IR and IP significantly increased 3 and 6 hours after reperfusion, LVESP, LVEF, LVFS, and MAP decreased significantly (P < 0.05); cTnT increased significantly in groups S, IR and IP 3 and 6 hours after reperfusion (P < 0.05). Compared with 3 hours after reperfusion, LVEDP and cTnT in group IR increased significantly 6 hours after reperfusion, LVESP, LVEF, LVFS, and MAP decreased significantly (P < 0.05). Compared with group S, LVEDP and cTnT in groups IR and IP were significantly higher 3 and 6 hours after reperfusion (P < 0.05), LVESP, LVEF, LVFS, and MAP were significantly lower (P < 0.05). Compared with group IR, LVEDP and cTnT in group IP were significantly lower 3 and 6 hours after reperfusion (P < 0.05), LVESP, LVEF, LVFS, and MAP were significantly higher (P < 0.05). There were no significantly differences between groups S and SP.
Conclusion Penehyclidine hydrochloride postconditioning can maintain hemodynamic stability, reduce cTnT concentration and improve cardiac function in rats with myocardial ischemia-reperfusion. |
