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| 星状神经节阻滞改善更年期大鼠抑郁行为及加速清除脑内损伤相关模式分子 |
| Stellate ganglion block ameliorates depression and accelerates the clearance of brain damage associated molecular patterns in menopausal rats |
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| DOI：10.12089/jca.2021.07.016 |
| 中文关键词: 星状神经节阻滞 更年期 抑郁 损伤相关模式分子 大鼠 |
| 英文关键词: Stellate ganglion block Menopause Depression Damage associated molecular patterns Rat |
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| 中文摘要: |
目的 探讨星状神经节阻滞（SGB）对更年期大鼠抑郁行为及脑内损伤相关模式分子（DAMPs）中主要分子浓度的影响。
方法 清洁级健康成年雌性SD大鼠40只，2月龄，体重180～220 g。将大鼠随机分为四组：对照组（C组），更年期大鼠抑郁模型组（M组），星状神经节注射生理盐水组（MN组）和星状神经节注射罗哌卡因组（MR组），每组10只。C组大鼠正常喂养；M组、MN组和MR组行双侧卵巢摘除术建立更年期大鼠模型，术后第8～28天行慢性束缚应激实验（CRS）建立更年期大鼠抑郁模型。术后第29～49天每日同一时点MN组于右侧星状神经节注射生理盐水0.2 ml，MR组于相同位置注射0.25%罗哌卡因0.2 ml。于手术当天、术后第7、14、21、28、35、42、49天行体重测量和糖水偏好实验，术后第28、35、42、49天行强迫游泳实验。术后第49天各项行为学测试结束后取大鼠海马组织，采用ELISA法检测DAMPs主要分子高迁移率族蛋白B1（HMGB1）、热休克蛋白70（HSP70）、S100钙结合蛋白B（S100B）、ATP和尿酸（UA）浓度。
结果 与C组比较，M组、MN组和MR组术后第7、14、21、28、35、42、49天体重明显减少（P＜0.05），糖水偏好百分比明显降低（P＜0.05），术后第28、35、42、49天强迫游泳不动时间明显延长（P＜0.05）；M组和MN组术后第49天海马组织HMGB1、HSP70、S100B、ATP浓度明显升高（P＜0.05）。与MN组比较，MR组术后第35、42、49天体重明显增加（P＜0.05），术后第42、49天糖水偏好百分比明显升高（P＜0.05），术后第35、42、49天强迫游泳不动时间明显缩短（P＜0.05），术后第49天海马组织HMGB1、HSP70、S100B、ATP浓度明显降低（P＜0.05）。
结论 星状神经节阻滞可以改善更年期大鼠抑郁行为，其机制可能与加速清除脑内DAMPs有关。 |
| 英文摘要: |
Objective To investigate the effects of stellate ganglion block (SGB) on depressive behavior and the concentrations of the main molecules of damage associated molecular patterns (DAMPs) in menopausal rats.
Methods Forty SPF-grade healthy adult SD female rats, aged 2 months, weighing 180-220 g, were randomly divided into four groups: control group (group C), menopausal rat depression model group (group M), model receiving stellate ganglion injection of normal saline group (group MN) and model receiving stellate ganglion injection of ropivacaine group (group MR), 10 rats in each group. Group C was fed normally; group M, group MN and group MR were given bilateral ovariectomy to establish menopausal rat model, and chronic restraint stress test (CRS) was performed to establish menopausal rat depression model on the 8th to 28th days after operation. From the 29th to 49th days after operation, 0.2 ml of normal saline was injected into the right stellate ganglion in group MN at the same time every day, while group MR were injected with 0.2 ml of 0.25% ropivacaine hydrochloride at the same position. Body weight and sucrose preference test were performed on the day of operation and on the 7th, 14th, 21st, 28th, 35th, 42nd and 49th days after operation. Forced swimming test was performed on the 28th, 35th, 42nd and 49th days after operation. On the 49th day after operation, the hippocampal tissues of rats were taken after the end of behavioral tests. The concentrations of main molecules of DAMPs including high mobility group protein B1 (HMGB1), heat shock protein 70 (HSP70), S100 calcium-binding protein B (S100B), adenosine triphosphate (ATP) and uric acid (UA) were detected by ELISA.
Results Compared with group C, the weight of rats and the percentage of sucrose preference in groups M, MN and MR decreased significantly on day 7, 14, 21, 28, 35, 42, 49 after operation (P < 0.05), and the time of immobility during forced swimming process delayed in groups M, MN and MR on day 28, 35, 42, 49 after operation (P < 0.05); the concentrations of HMGB1, HSP70, S100B, and ATP in hippocampus on 49th day after operation in groups M and MN increased significantly (P < 0.05). Compared with group MN, the weight of rats increased significantly on 35th, 42nd and 49th days after operation (P < 0.05), the sucrose preference percentage increased markedly on the 42nd and 49th days after operation (P < 0.05), and the immobility time of forced swimming on the 35th, 42nd and 49th days after operation was also shortened (P < 0.05), the concentrations of HMGB1, HSP70, S100B and ATP in hippocampus were significantly reduced on 49th day (P < 0.05).
Conclusion Stellate ganglion block can ameliorate depression in menopausal rats, and its mechanism may be related to accelerating the clearance of DAMPs in the brain of menopausal depressive rats. |
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