Objective To observe the effect of infusion of different storage time suspended red blood cells on endotoxin shock-induced acute lung injury in rabbits.
Methods Forty-eight clean male New Zealand rabbits of six months old, weighing 2.0-2.5 kg, were divided into 6 groups (n = 8 for each group) using a random number table method: sham-operated group (group S), endotoxin shock group (group ES), endotoxin shock + storage time of infusing red blood cells 1 day group (group R0), 1 week group (group R1), 2 weeks group (group R2), and 3 weeks group (group R3). In groups ES, R0, R1, R2, and R3, 15% blood volume were collected from ear central artery of each rabbit 1 day before the experiment. The next day, endotoxin shock model was induced by injecting lipopolysaccharide through the ear vein, group S was given the same amount of saline. After intravenous injection of LPS or saline for 2 hours, group S and group ES were given an appropriate amount of Sodium Lactate Ringer's solution to maintain MAP > 65 mmHg. Groups R0, R1, R2, and R3 were infused with red blood cells stored for different time, and the infusion volume was 8 ml. After the infusion, an appropriate amount of Sodium Lactate Ringer's solution was added to maintain MAP > 65 mmHg. After intravenous injection of LPS for 6 hours, serum concentrations of interleukin-1β (IL-1β), interleukin-8 (IL-8), tumor Necrosis Factor-α (TNF-α) in each group were detected by ELISA. Lung tissues were collected from sacrificed rabbits and then stained with haematoxylin, Histopathology (HE), pathological changes, lung injury score and wet-to-dry weight ratios (W/D) were assessed; concentrations of malondialdehyde (MDA), the myeloperoxidase (MPO) activity, and the activity of superoxide dismutase (SOD) in the lung tissue were detected.
Results Compared with group S, the serum concentrations of IL-1β, IL-8 and TNF-α were significantly increased, lung injury score and W/D ratio were significantly increased, the activity of MPO and concentration of MDA were significantly up-regulated, the activity of SOD was significantly decreased in groups ES, R0, R1, R2 and R3 (P < 0.05). Compared with group ES, the serum concentrations of IL-1β, IL-8 and TNF-α were significantly decreased, lung injury score and W/D ratio were significantly decreased, the activity of MPO and concentration of MDA were significantly decreased, the activity of SOD was significantly up-regulated in groups R0, R1, R2 and R3 (P < 0.05). Compared with group R0, the concentrations of IL-8 was significantly increased, lung injury score and W/D ratio were significantly increased, the activity of MPO and concentration of MDA were significantly up-regulated, the activity of SOD was significantly decreased in group R1 and group R2 (P < 0.05); the concentrations of IL-1β, IL-8 and TNF-α were significantly increased, lung injury score and W/D ratio were significantly increased, the expression of activity of MPO and concentration of MDA were significantly up-regulated, the activity of SOD was significantly decreased in group R3 (P < 0.05). Compared with group R1, the concentration of IL-8 was significantly increased, lung injury score and W/D ratio were significantly increased, the concentration of MDA were significantly up-regulated, the activity of SOD were significantly decreased in group R2 (P < 0.05); the concentrations of IL-1β, IL-8 and TNF-α were significantly increased, lung injury score and W/D ratio were significantly increased, the activity of MPO and concentration of MDA were significantly up-regulated,the activity of SOD was significantly decreased in group R3 (P < 0.05). Compared with group R2, the serum concentrations of IL-1β, IL-8 and TNF-α were significantly increased, lung injury score and W/D ratio were significantly increased, the activity of MPO and concentration of MDA were significantly up-regulated, the activity of SOD was significantly decreased in group R3 (P < 0.05).
Conclusion Transfusion of red blood cells with different storage time can improve acute lung injury in endotoxin shock rabbits, and the red blood cells stored for no longer than 2 weeks were more effective in treating acute lung injury in rabbits with endotoxin shock. |
