文章摘要
右美托咪定改善异氟醚所致成年小鼠早期认知功能障碍及对NONMMUT000984表达的影响
Dexmedetomidine improves isoflurane-induced early cognitive dysfunction in adult mice and its effect on lncRNA NONMMUT000984 expression
  
DOI:10.12089/jca.2020.12.015
中文关键词: 右美托咪定  NONMMUT000984  早期认知功能障碍
英文关键词: Dexmedetomidine  NONMMUT000984  Early cognitive dysfunction
基金项目:2016军队后勤科研项目(CCD16J001)
作者单位E-mail
黄青青 610083,成都市,西部战区总医院麻醉科  
袁利邦 610083,成都市,西部战区总医院麻醉科  
巩固 610083,成都市,西部战区总医院麻醉科 gonggu68@163.com 
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中文摘要:
      
目的 观察右美托咪定对异氟醚所致成年小鼠早期认知功能障碍及长链非编码RNA(lncRNA)NONMMUT000984表达的影响。
方法 雄性C57/BL6小鼠160只,8周龄,体重20~23 g,随机分为四组,每组40只:对照组(C组),不做任何处理;异氟醚组(I组),持续吸入1.4%异氟醚4 h;生理盐水组(NI组),麻醉前30 min腹腔注射与右美托咪定等体积的生理盐水+持续吸入1.4%异氟醚4 h;右美托咪定组(DI组)麻醉前30 min腹腔注射右美托咪定25 μg/kg+持续吸入1.4%异氟醚4 h。麻醉后3、7 d采用旷场实验和Morris水迷宫实验测定认知功能,QT-PCR法测定海马组织中NONMMUT000984表达量,Western blot法测定海马组织中PSD-95与SYN-1蛋白含量,HE染色观察海马区神经元细胞形态。
结果 麻醉后3、7 d,与C组和DI组比较,I组和NI组活动总路程明显缩短,站立次数和穿越平台次数明显减少,NONMMUT000984表达量明显降低,逃避潜伏期明显延长,SYN-1和PSD-95蛋白含量明显升高(P<0.05);海马神经元细胞排列紊乱,层次不清,细胞质减少。麻醉后3、7 d,I组和NI组活动总路程、站立次数、穿越平台次数、逃避潜伏期、NONMMUT000984表达量、SYN-1和PSD-95蛋白含量差异无统计学意义。
结论 右美托咪定可通过增加成年小鼠突触可塑性相关蛋白表达来改善异氟醚麻醉所致的早期学习记忆障碍,同时可降低NONMMUT000984表达量。
英文摘要:
      
Objective To observe the effect of dexmedetomidine on isoflurane-induced early cognitive dysfunction and expression of NONMMUT000984 in adult mice.
Methods A total of 160 male C57/BL6 mice, aged 8 weeks, weighing 20-23 g, were randomly divided into 4 groups with 40 mice in each group. The control group (group C) was untreated, the isoflurane group (group I) continued to inhale 1.4% isoflurane for 4 h, the saline group (group NI) was injected intraperitoneally with equal volume of saline with dexmedetomidine 30 min before anesthesia and continued to inhale 1.4% isoflurane for 4 h, and the dexmedetomidine group (group DI) was injected intraperitoneally with dexmedetomidine 25 μg/kg 30 min before anesthesia and continued to inhale 1.4% isoflurane for 4 h. The cognitive function was measured by open field test and Morris water maze test, the expression of NONMMUT000984 in hippocampal tissue was measured by QT-PCR method, the content of PSD-95/SYN-1 protein in hippocampal tissue was determined by Western blot, and the morphology of hippocampal neurons was observed by HE staining 3 and 7 days after anesthesia.
Results Compared with group C and group DI, mice in groups I and NI had a significant decrease in the total distance within 5 minutes, the number of standing, the number of crossing platforms, and the relative expression of NONMMUT000984 on day 3 and day 7 after anesthesia (P < 0.05); the escape latency, SYN-1 and PSD-95 protein content increased significantly in groups I and NI (P < 0.05),the hippocampal neuronal cells were disorderly arranged, the layers were unclear, and the cytoplasm decreased in groups I and NI (P < 0.05). There was no significant difference in the total distance within 5 minutes, the number of crossing platforms, the escape latency, the relative expression of NONMMUT000984, the relative protein content of SYN-1 and PSD-95, cell arrangement and morphology between group I and group NI on day 3 and day 7 after anesthesia.
Conclusion Dexmedetomidine can increase the expression of synaptic plasticity-related proteins in adult mice, improve early learning and memory impairment caused by isoflurane anesthesia, and reduce the expression of NONMMUT000984.
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