Ojective To investigate the effects of congestion preconditioning on intestinal and liver injury caused by intestinal congestion-reperfusion in rabbit model of hepatic portal vein occlusion.
Methods Seventy-five male Japanese big eared rabbits were selected， weighing 2.5-5.0 kg, were divided into five groups with 15 rabbits in each group: sham operation group (group S), congestion-reperfusion group (group OC), preconditioning group A (group OC5), preconditioning group B (group OC10) and preconditioning C group (group OC15). Group S only underwent laparotomy to expose the first porta hepatis for 30 min. In group OC, portal vein was opened after portal vein occluded 30 min. Preconditioning groups were divided into group OC5 (clamping 5 min and open 5 min), group OC10 (clamping 10 min and open 10 min), and group OC15 (clamping 15 min and open 15 min) according to different portal vein occlusion time, then portal vein was occluded for 30 min and then opened. The serum concentrations of malondialdehyde (MDA), endotoxin, tumor necrosis factor-α (TNF-α), and alanine aminotransferase (ALT) were detected in portal vein 2, 8, 24 h after operation. The blood sample were collected 24 h after operation, the rabbits were anesthetized again and sacrificed to obtained the intestinal mucosa of terminal ileum and liver tissue, and the pathological morphological change was observed under the microscope.
Results Compared with group S, the serum concentrations of MDA, endotoxin, TNF-α, and ALT in groups OC, OC5, OC10, and OC15 were increased significantly 2, 8, and 24 h after surgery (P ＜ 0.05), respectively. Compared with group OC, the serum concentrations of MDA, endotoxin, TNF-α, and ALT in groups OC5, OC10, and OC15 were significantly reduced 2, 8, and 24 h after operation, respectively (P ＜ 0.05). Compared with group OC5, the serum concentrations of MDA, TNF-α, and ALT in group OC10 were significantly reduced 2, 8, and 24 h after surgery (P ＜ 0.05). Compared with group OC5, the serum concentrations of endotoxin in group OC10 decreased significantly 2 and 8 h after operation (P ＜ 0.05). Compared with group OC10, the serum concentrations of MDA and ALT in group OC15 were significantly increased 8 h after operation (P ＜ 0.05). Compared with group S, the Chiu score of intestinal mucosal injury in groups OC, OC5, OC10, and OC15 was significantly increased, respectively (P ＜ 0.05). Compared with group OC, the Chiu score of mucosal injury in groups OC5, OC10 and OC15 were significantly reduced (P ＜ 0.05). Compared with group OC5, the Chiu score of intestinal mucosal injury in groups OC10 and OC15 were significantly reduced (P ＜ 0.05).
Conclusion Congestion preconditioning can reduce intestinal congestion-reperfusion injury caused by hepatic portal vein occlusion, reduce endotoxemia and liver injury caused by excessive inflammatory reaction and reactive oxygen species. |
