|
| 伤害性杏仁核中mGluR5对芬太尼诱导痛觉过敏大鼠杏仁核神经通路兴奋性突触传递的影响 |
| Effect of mGluR5 in nociceptive amygdala on excitatory synaptic transmission in amygdaloid neural pathway in rats with fentanyl-induced hyperalgesia |
| |
| DOI：10.12089/jca.2020.07.015 |
| 中文关键词: 痛觉过敏 代谢型谷氨酸受体5 杏仁核 芬太尼 神经通路 |
| 英文关键词: Hyperalgesia Metabolic glutamate receptor 5 Amygdala Fentanyl Neural pathway |
| 基金项目:国家自然科学基金(81771196) |
|
| 摘要点击次数: 1726 |
| 全文下载次数: 424 |
| 中文摘要: |
目的 探讨伤害性杏仁核(CeLC)中代谢型谷氨酸受体5(mGluR5)对阿片诱导痛觉过敏(OIH)大鼠杏仁核神经通路兴奋性突触传递的影响。
方法 实验一: 取SD雄性大鼠18只，随机分为对照组（C组）、痛觉过敏+DMSO组（OIH+DMSO组）和痛觉过敏+mGluR5选择性拮抗剂组(OIH+MTEP组)，每组6只。三组大鼠均接受右侧CeLC置管，恢复1周后OIH+DMSO组和OIH+MTEP组经皮下注射芬太尼造OIH模型，芬太尼剂量为60 μg/kg，4次，间隔时间15 min，C组皮下注射等容量生理盐水。6.5 h后OIH+DMSO组大鼠CeLC区注射10%二甲基亚砜（DMSO）0.5 μl；C组和OIH+MTEP组大鼠CeLC区注射MTEP 15 μg。观察造模前(T0)、造模后（T1）和给药后(T2)大鼠机械缩足阈值(WMT)和热缩足反射潜伏期(TWL)。实验二: 另取10只大鼠随机分为正常组（N组）和痛觉过敏组（OIH组），OIH组注射芬太尼造OIH模型，N组注射等容量生理盐水。使用双电极膜片钳技术分别记录使用MTEP前后两组大鼠基底外侧杏仁核(BLA)-CeLC神经通路刺激诱发的兴奋性突触后电流(eEPSCs)的幅值。
结果 实验一：与T0时比较，T1时OIH+DMSO组与OIH+MTEP组大鼠WMT明显降低、TWL明显缩短(P<0.05)；与T1比较，T2时OIH+MTEP组大鼠WMT明显升高、TWL明显延长(P<0.05)；T2时OIH+MTEP组WMT明显高于OIH+DMSO组，TWL明显长于OIH+DMSO组(P<0.05)，T2时OIH+MTEP组和C组WMT及TWL差异无统计学意义。实验二：与OIH组使用MTEP前比较，OIH组使用MTEP后eEPSCs幅值明显降低。N组使用MTEP前和N组使用MTEP后eEPSCs幅值差异无统计学意义。OIH组使用MTEP前eEPSCs幅值明显高于N组使用MTEP前(P<0.05)。
结论 激活CeLC中mGluR5可通过增强BLA-CeLC神经通路的兴奋性突触传递来参与调控芬太尼诱导的痛觉过敏。 |
| 英文摘要: |
Objective To investigate the effect of metabolic glutamate receptor 5 (mGluR5) in nociceptive amygdala (the laterocapcular division of central amygdala, CeLC) on excitatory synaptic transmission in amygdaloid neural pathway in rats with opioid-induced hyperalgesia (OIH).
Methods Experiment 1∶18 male SD rats were randomly divided into the control group (group C), the hyperalgesia + DMSO group (group OIH+DMSO) and the hyperalgesia + mGluR5 selective antagonist group (group OIH+MTEP), with 6 rats in each group. All rats in the three groups received CeLC catheterization on the right side. After one week of recovery, group OIH+DMSO and group OIH+MTEP received subcutaneous injection of fentanyl to establish OIH model. The dose of fentanyl was 60 μg/kg, 4 times, with an interval of 15 min. Group C was subcutaneously injected with saline of equal volume.After 6.5 h, rats in the group OIH+DMSO were injected with 10% DMSO 0.5 μl in the CeLC. Rats in the group C and group OIH+MTEP were injected with MTEP 15 μg in the CeLC. The changes of mechanical withdrawal threshold (WMT) and thermal withdrawal latency (TWL) were tested pre-OIH (T0), post-OIH (T1), and post-drug (T2). Experiment 2: another 10 rats were randomly divided into 2 groups: the normal group (group N) and the hyperalgesia group (group OIH). Group OIH was injected with fentanyl to make OIH model, and group N was injected with normal saline of equal volume. The amplitude of evoked excitatory postsynaptic currents (eEPSCs) of the basolateral amygdala (BLA)-CeLC neural pathway before and after administration of MTEP was recorded by using double electrode patch clamp technique.
Results Experiment 1: Compared with T0,WMT and TWL of rats in group OIH+DMSO and group OIH+MTEP were significantly decreased and shortened at T1. Compared with T1, WMT was significantly increased and TWL was significantly prolonged in rats of group OIH+MTEP at T2. Compared with group OIH + DMSO, WMT was significantly increased and TWL was significantly prolonged in group OIH+MTEP at T2 (P < 0.05). There was no significant difference in WMT and TWL between group OIH + MTEP and group C at T2. Experiment 2: Compared with the group OIH pre-MTEP, the amplitude of eEPSCs in the group OIH was significantly decreased post-MTEP. There was no significant difference in eEPSCs amplitude between group N pre-MTEP and group N post-MTEP. The amplitude of eEPSCs in group OIH pre-MTEP was significantly higher than group N pre-MTEP (P < 0.05).
Conclusion Activation of mGluR5 in the CeLC may be involved in the regulation of fentanyl-induced hyperalgesia by enhancing excitatory synaptic transmission in the BLA-CeLC neural pathway. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
