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| 冷缺血期氢混合气膨肺对大鼠肺移植后的能量代谢保护作用 |
| Protective effect of hydrogen mixture on energy metabolism of rat lung grafts during cold ischemia |
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| DOI：10.12089/jca.2020.05.013 |
| 中文关键词: 氢混合气 膨肺 肺移植 冷缺血期 能量代谢 线粒体 |
| 英文关键词: Hydrogen mixture Expanded lung Lung transplantation Cold ischemia Energy metabolism Mitochondria |
| 基金项目:国家自然科学基金（81570088） |
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| 摘要点击次数: 2117 |
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| 中文摘要: |
目的 探讨冷缺血期氢混合气膨肺对大鼠肺移植后能量代谢的保护作用。
方法 健康雄性SD大鼠42只，8～10周龄，体重250～300 g，随机取21只大鼠为供体大鼠，其余21只为受体大鼠，采用随机数字法将两组大鼠进一步分为三个亚组（每组7只）：对照组、氧气组和氢气组。冷缺血期对照组不使用气体膨肺，氧气组采用40% O2 + 60% N2膨肺，氢气组采用3% H2 + 40% O2 + 57% N2膨肺，2 h后肺移植。受体大鼠于肺移植前、再灌注3 min、1、2 h时行动脉血气分析检测PaO2、PaCO2、pH、碱剩余（BE），计算氧合指数（OI），留取血清与移植肺组织，术后2 h采用ELISA法检测血清白介素-8（IL-8）、白介素-10（IL-10）、肿瘤坏死因子α（TNF-α）浓度。肺组织HE染色，记录肺组织损伤评分（LIS）。采用ELISA法检测肺组织中乳酸（Lac）、丙酮酸（PA）、三磷酸腺苷（ATP）浓度，采用透射电镜观察肺线粒体结构。
结果 与对照组比较，再灌注2 h氢气组、氧气组pH、BE、OI明显升高（P<0.05）；与对照组比较，氢气组、氧气组血清IL-8、TNF-α浓度明显降低，IL-10浓度明显升高（P<0.05）。与氧气组比较，再灌注2 h氢气组OI、pH、BE明显升高（P<0.05）；与氧气组比较，氢气组血清IL-8、TNF-α浓度明显降低，IL-10浓度明显升高（P<0.05）。LIS评分氢气组明显高于氧气组（P<0.05）。对照组移植肺组织肺泡中有大量透明膜形成，肺泡内出血明显，肺泡隔和肺泡重度水肿，大量中性粒细胞浸润；氢气组、氧气组移植肺组织病理损伤程度轻于对照组，氢气组轻于氧气组。与对照组比较，氧气组和氢气组受体大鼠移植肺组织中Lac、PA浓度明显降低（P<0.05），ATP浓度明显升高（P<0.05），移植肺组织线粒体的面积、周长、费雷特径明显增大（P<0.05），线粒体圆度明显降低（P<0.05），嵴紊乱比例明显小于氧气组（P<0.05）。
结论 冷缺血期氢混合气膨肺能提高大鼠移植肺氧合功能，降低乳酸/丙酮酸比值，减轻炎症反应，部分改善肺损伤，提高组织ATP含量，保护肺线粒体，对移植肺的能量代谢功能具有保护作用，这种效果可能是通过对线粒体保护作用而产生的。 |
| 英文摘要: |
Objective To discuss cold ischemia phase hydrogen, mixture of lung on rat lung grafts and energy metabolism.
Methods Forty-two healthy male SD rats, aged 8-10 weeks, weighing 250-300 g, were randomly divided into three groups: control group without the use of gas, oxygen group using 40% O2 + 60% N2, hydrogen group using 3% H2 + 40% O2 + 57% N2. lung transplantation receptor in rats after 2 h reperfusion 3 min, 1 h and 2h before transplantation. Blood gas analysis was performed on the arterial blood and detection of PaO2, PaCO2, pH value, base residual (BE) value, calculation of OI, serum was collected and transplanted into lung tissue, and serum interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-α) and lung tissue HE staining were observed to calculate lung injury store (LIS). Lung tissue lactic acid (Lac), pyruvic acid (PA), adenosine triphosphate(ATP) content and statistical pulmonary mitochondria structure change were dected under the electron microscope.
Results Compared with the control group, the pH value of OI in the hydrogen group and the oxygen group was significantly increased 2 h after perfusion (P < 0.05). The concentration of serum il-8 tnf-alpha was significantly decreased, and the concentration of IL-10 was significantly increased (P < 0.05). Compared with the oxygen group, the pH value of OI was significantly increased after 2 h of reperfusion in the hydrogen group (P < 0.05). The concentration of serum IL-8 TNF-α was significantly decreased and that of IL-10 was significantly increased (P < 0.05). LIS score in the hydrogen group was significantly higher than that in the oxygen group (P < 0.05). In the control group, there was a large amount of transparent membrane formation in the alveoli of the transplanted lung tissue, significant intraalveolar hemorrhage, severe alveolar septum and alveolar edema, and a large number of neutrophils infiltration. In the hydrogen group and the oxygen group, the degree of pathological damage of the transplanted lung tissue was less than that of the control group, and the hydrogen group was lighter than that of the oxygen group compared with the control group. The content of LAC PA in the transplanted lung tissue of the oxygen group and the hydrogen group was significantly lower (P < 0.05), and the content of ATP was significantly higher (P < 0.05). The area circumference of mitochondria in the transplanted lung was significantly increased (P < 0.05), the roundness of mitochondria was significantly decreased (P < 0.05), and the proportion of cristae disorder was significantly lower than that in the oxygen group (P < 0.05).
Conclusion Swelling the lungs with hydrogen mixture swelling in cold ischemia can improve lung oxygenation, reduce lactic acid/pyruvate, reduce inflammation, partially improve lung injury, increase tissue ATP content, protect lung mitochondria, and energy metabolism of transplanted lung. It has a protective effect through protecting. |
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