文章摘要
lncRNA-MALAT1/miRNA-145在舒芬太尼预处理减轻大鼠心肌缺血-再灌注损伤中的作用
Effects of lncRNA-MALAT1/miRNA-145 on sufentanil pretreatment alleviating myocardial ischemia-reperfusion injury in rats
  
DOI：10.12089/jca.2020.04.016
中文关键词: 舒芬太尼  心肌缺血-再灌注损伤  肺腺癌转录子1  miRNA-145
英文关键词: Sufentanil  Myocardial ischemia-reperfusion injury  MALAT1  miRNA-145
基金项目:内蒙古自治区科技计划项目(201702118);内蒙古自治区人民医院院内基金(2016014)
作者单位E-mail
赵智慧 010017,呼和浩特市,内蒙古自治区人民医院麻醉科 zzh19880101@126.com 
白香花 010017,呼和浩特市,内蒙古自治区人民医院麻醉科  
何金玲 010017,呼和浩特市,内蒙古自治区人民医院麻醉科  
段伟琴 010017,呼和浩特市,内蒙古自治区人民医院麻醉科  
丁辰颖 010017,呼和浩特市,内蒙古自治区人民医院麻醉科  
夏中元 武汉大学人民医院麻醉科  
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中文摘要:
      
目的 探讨长链非编码RNA(lncRNA)肺腺癌转录子1(MALAT1)在舒芬太尼预处理减轻大鼠心肌缺血-再灌注损伤(MIRI)中的作用。
方法 SPF级大鼠36只,体重180～200 g,随机分为三组:假手术组(Sham组),缺血-再灌注损伤组(IR组),缺血-再灌注损伤+舒芬太尼组(SUF组),每组12只。IR组和SUF组建立大鼠心肌缺血-再灌注损伤模型,SUF组于再灌注前10 min尾静脉注射舒芬太尼1 μg/kg,Sham组和IR组注射等体积生理盐水。再灌注后2 h检测大鼠血清中肌酸激酶同工酶MB(CK-MB)、心肌肌钙蛋白T(cTnT)和乳酸脱氢酶(LDH)活性,同时检测大鼠心肌梗死面积;检测心肌细胞凋亡相关蛋白Bcl-2、Bax、Cleaved-caspase-3和Caspase-3含量;检测大鼠心肌组织中lncRNA-MALAT1和miRNA-145的相对表达量。
结果 与Sham组比较,IR组和SUF组CK-MB和cTnT浓度明显升高,LDH活性明显增强,心肌梗死面积明显增大,心肌组织中Bax/Bcl-2比值明显降低,Cleaved-caspase-3蛋白含量明显升高,lncRNA-MALAT1相对表达量明显升高,而miRNA-145相对表达量明显降低(P<0.05)。与IR组比较,SUF组CK-MB和cTnT浓度明显降低,LDH活性明显减弱,心肌梗死面积明显减小,Bax/Bcl-2比值明显升高,Cleaved-caspase-3蛋白含量明显降低,lncRNA-MALAT1相对表达量明显降低,miRNA-145相对表达量明显增加(P<0.05)。
结论 舒芬太尼预处理能减轻大鼠心肌缺血-再灌注损伤程度,其机制可能与抑制lncRNA-MALAT1、升高miRNA-145表达有关。
英文摘要:
      
Objective To investigate the effect of sufentanil pretreatment on myocardial ischemia-reperfusion injury (MIRI) in rats, and to explore its mechanism of action from lncRNA-MALAT1/miRNA-145.
Methods Thirty-six SPF rats of 180-200 g were randomly divided into three groups: sham operation group (group Sham), ischemia-reperfusion injury group (group IR), and ischemia-reperfusion injury+sufentanil group (group SUF), n = 12 in each group. Group IR and group SUF established a rat model of MIRI. In group SUF, sufentanil 1 μg/kg was injected into the tail vein at 10 min before reperfusion, whereas the other two groups were injected with an equal volume of saline. The serum concentration of creatine kinase isoenzyme MB (CK-MB) activity, cardiac troponin T (cTnT) and lactate dehydrogenase (LDH) were detected at 2 h after reperfusion. The area of myocardial infarction (MI) was detected. The expression of cardiomyocyte apoptosis-related proteins Bcl-2, Bax, Cleaved-caspase-3 and Caspase-3 were detected using Western blot. Relative expression of lncRNA-MALAT1 and miRNA-145 in myocardial tissue of rats were investigated by RT-PCR.
Results Compared with group Sham, the concentrations of CK-MB and cTnT, the area of MI and LDH activity were increased significantly, Bax/Bcl-2 ratio was significantly decreased and Cleaved-caspase-3 protein expression was significantly increased, the relative expression of lncRNA-MALAT1 was up-regulated, whereas miRNA-145 expression was down-regulated in group IR and group SUF (P < 0.05). Compared with group IR, the concentrations of CK-MB and cTnT, the area of MI and LDH activity were decreased, Bax/Bcl-2 ratio was increased and Cleaved-caspase-3 protein expression was significantly decreased, the relative expression of lncRNA-MALAT1 was down-regulated, whereas miRNA-145 expression was up-regulated significantly in group SUF (P < 0.05).
Conclusion Sufentanil pretreatment can alleviate the degree of myocardial ischemia-reperfusion injury in rats, and its mechanism may be related to the regulation of lncRNA-MALAT1/miRNA-145 expression.
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