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| 远端肢体缺血预处理对氯胺酮致发育期大鼠海马CA1区神经细胞Bcl-2和Bax表达失衡的影响 |
| Effects of remote limb ischemic preconditioning on the imbalance of Bcl-2 and Bax in hippocampal CA1 neurons of neonatal rats during ketamine anesthesia |
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| DOI：10.12089/jca.2020.02.015 |
| 中文关键词: 氯胺酮 远端肢体缺血预处理 发育期大鼠 B淋巴细胞瘤-2 Bcl-2相关X蛋白 |
| 英文关键词: Ketamine Remote limb ischemic preconditioning Developmental rats B cell lymphoma-2 Bcl-2 assaciated X protein |
| 基金项目:国家自然科学基金(81560225);宁夏自然科学基金(NZ15143) |
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| 中文摘要: |
目的 研究远端肢体缺血预处理对氯胺酮麻醉导致的发育期大鼠海马CA1区神经细胞抗凋亡因子B淋巴细胞瘤-2（Bcl-2）和凋亡因子Bcl-2相关X蛋白（Bax）表达失衡的影响。
方法 SD新生大鼠48只,5日龄,体重8～12 g,随机分为四组:对照组(C组)于出生第7天腹腔注射生理盐水8 ml／kg,间隔2 h注射一次,共注射6次;远端肢体缺血预处理组(RIPC组)于出生第5天行右后肢缺血5 min,再灌注5 min预处理,4个循环,48 h后腹腔注射生理盐水8 ml/kg,方法同C组;氯胺酮组(K组)于出生第7天腹腔注射氯胺酮20 mg／kg,方法同C组;肢体缺血预处理+氯胺酮组(RK组)于出生第5天行远端肢体缺血预处理,48 h后腹腔注射氯胺酮20 mg/kg,方法同C组。四组于腹腔注射完成后12 h经心脏灌注取脑,分别采用免疫组化法和Western blot法观察大鼠海马CA1区凋亡因子Bax和抗凋亡因子Bcl-2阳性细胞数量和蛋白含量。
结果 与RIPC组比较,K组和RK组海马CA1区神经细胞Bax阳性细胞数量明显增多，蛋白含量明显升高（P<0.05），Bcl-2阳性细胞数量明显减少，蛋白含量明显降低(P＜0.05)。C组和RIPC组海马CA1区神经细胞Bcl-2和Bax阳性细胞数量和蛋白含量差异无统计学意义。与K组比较,RK组海马CA1区神经细胞Bax阳性细胞数量明显减少，蛋白含量明显降低（P<0.05），Bcl-2阳性细胞数量明显增多，蛋白含量明显升高(P＜0.05)。
结论 远端肢体缺血预处理可减轻氯胺酮大剂量、多次注射导致的发育期大鼠海马CA1区神经细胞Bcl-2、Bax表达失衡。 |
| 英文摘要: |
Ojective To study the effects of remote limb ischemic preconditioning (RIPC) on the expression imbalance of B cell lymphoma-2 (Bcl-2) and B cell lymphoma-2 assaciated X protein (Bax) in hippocampal CA1 neurons of the neonatal rats under ketamine anesthesia.
Methods Forty-eight Sprague-Dawley rats on postnatal day 5, weighing 8-12 g, were randomly divided into four groups: control group (group C), remote limb ischemic preconditioning group (group RIPC), ketamine group (group K), remote limb ischemic preconditioning + ketamine group (group RK). In group C, normal saline 8 ml／kg was intraperitoneally injected on postnatal day 7, 6 times at an interval of 2 hours. Rats in group RIPC were subjected to limb ischemia for 5 minutes and reperfusion for 5 minutes for 4 cycles on postnatal day 5, and then injected with the same volume of saline after 48 hours. Rats in group K were intraperitoneally injected with ketamine 20 mg／kg instead of normal saline, once every 2 hours after administration for 6 times. Rats in group RK were subjected to remote ischemic preconditioning on postnatal day 5 and underwent the same treatment as group K after 48 hours. Rats in the four groups were sacrificed to cardiac perfusion on postnatal day 9. The changes of Bcl-2 and Bax expression in the hippocampal CA1 were observed by immunohistochemistry and western blot.
Results Compared with group RIPC, expression of Bax in groups K and RK was significantly increased while Bcl-2 expression was significantly decreased (P ＜ 0.05). There was no significant change in the expression of Bcl-2 and Bax between group RIPC and group C. Compared with group K, Bax in group RK was significantly decreased while Bcl-2 expression was significantly increased (P ＜ 0.05).
Conclusion Remote limb ischemic preconditioning reduces the imbalance of Bcl-2, Bax in the hippocampal CA1 region induced by multiple exposure of ketamine in developmental rats. |
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