|
| 七氟醚对新生小鼠fractalkine/CX3CR1表达及学习记忆功能的影响 |
| Effects of sevoflurane on expression of fractalkine and CX3CR1 and learning and memory function in developing mouse brain |
| |
| DOI：10.12089/jca.2020.01.015 |
| 中文关键词: CX3CR1 Fractalkine 七氟醚 学习记忆 发育期大脑 |
| 英文关键词: CX3CR1 Fractalkine Sevoflurane Learning and memory Developed brain |
| 基金项目:内蒙古自治区人民医院博士科研启动基金（BS201511） |
|
| 摘要点击次数: 3489 |
| 全文下载次数: 921 |
| 中文摘要: |
目的 探讨七氟醚对新生小鼠fractalkine/CX3CR1表达及学习记忆功能的影响。
方法 取健康6日龄C57BL/6新生小鼠36只,分为对照组(C组)、七氟醚1组(S1组)、七氟醚2组(S2组),每组12只。C组小鼠出生后第6、7和8天每天接受1次2 h的 60％氧浓度的吸入;S1组小鼠出生后第6、7和8天每天接受1次2 h的2％七氟醚麻醉,吸入氧浓度为60％;S2组小鼠出生后第6、7和8天每天接受1次2 h的3％七氟醚麻醉,吸入氧浓度为60％。三组分别于出生后第9天(T1)及出生后第34天(T2)时各处死6只小鼠,取脑组织,采用Western blot法检测海马脑片fractakine和CX3CR1蛋白含量;real-time PCR检测海马脑片fractalkine和CX3CR1 mRNA的表达量。三组剩余6只小鼠用药3周后即出生第29天时行为期5 d的mirror水迷宫实验进行学习记忆能力测试并记录。记录出生后第29～32天小鼠的逃避潜伏期和游泳速度、原平台穿越次数。
结果 与C组比较,S1组和S2组学习记忆功能降低且海马脑片fractalkine和CX3CR1蛋白含量和mRNA表达量明显降低(P<0.05);与S1组比较,S2组学习记忆功能降低且海马脑片fractalkine和CX3CR1蛋白含量和mRNA表达量明显降低(P<0.05)。
结论 6日龄小鼠连续吸入七氟醚3 d,3周后出现七氟醚麻醉浓度递增的学习记忆能力障碍。小鼠吸入七氟醚可致幼龄小鼠海马脑片fractalkine、CX3CR1蛋白含量和mRNA表达量降低。 |
| 英文摘要: |
Objective To investigate the effects of sevoflurane on expression of fractalkine,CX3CR1 and learning and memory function in developing mouse brain.
Methods Thirty-six healthy C57BL/6 newborn mice aged 6 days were randomly divided into three groups: control group (group C), sevoflurane 1 group (group S1) and sevoflurane 2 group (group S2), 12 rats in each group. The mice in group C received 60％ oxygen inhalation 2 h daily for 3 days. The mice in group S1 received the mixture of 2％ sevoflurane and 60％ oxygen 2 hours daily for 3 days. The mice in group S2 were anesthetized with the mixture of 3％ sevoflurane and 60％ oxygen 2 hours daily for 3 days. Six mice were sacrificed on the 9th day (T1) and 34th day (T2) after birth, respectively,brain tissues were taken and the protein and mRNA expression of fractalkine and CX3CR1 in hippocampal slices was detected by Western blot and realtime PCR. Three weeks after medication (on the 29th day of birth) the learning and memory abilities in each group were tested by mirror water maze test for 5 days. The escape latency and swimming speed were recorded on the 29th 32th day after birth,and the times of crossing the original platform were recorded on the 33th day after birth.
Results Compared with group C, the learning and memory function was decreased,and the protein and gene expression of fractalkine and CX3CR1 in hippocampal slices of groups S1 and S2 were decreased (P<0.05). Compared with group S1, the learning and memory function was decreased, so were the protein and gene expression of fractalkine and CX3CR1 in hippocampal slices of group S2 (P<0.05).
Conclusion Sevoflurane anesthesia could cause in concentration-increased learning and memory disorders in 6-day old mouse. Sevoflurane treatment could decreased the level of fractalkine and CX3CR1 in hippocampal slices of young mice. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
