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| 脉冲射频治疗对带状疱疹后神经痛模型大鼠脊髓背角自噬的影响 |
| Effect of pulsed radiofrequency on autophagy in rat spinal cord in postherpetic neuralgia model |
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| DOI：10.12089/jca.2019.12.016 |
| 中文关键词: 脉冲射频 带状疱疹后神经痛 脊髓 自噬 |
| 英文关键词: Pulsed radiofrequency Postherpetic neuralgia Spinal cord Autophagy |
| 基金项目:广西自然科学基金(2017GXNSFAA10027) |
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| 摘要点击次数: 3259 |
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| 中文摘要: |
目的 观察脉冲射频(PRF)治疗对带状疱疹后神经痛(PHN)模型大鼠疼痛的影响,探讨脊髓背角自噬改变的可能作用。
方法 雄性SD大鼠60只,体重200～240 g,随机分为五组：空白对照组(Blank组)、溶剂组(Solvent组)、PHN模型组(PHN组)、假治疗组(Sham组)和治疗组(PRF组),每组12只。PHN组、Sham组及PRF组腹腔注射树脂毒素(RTX)0.2 g/kg制备PHN模型。Blank组腹腔注射与PHN组相同体积的生理盐水。Solvent组腹腔注射相同体积的溶剂（10%吐温80、10%乙醇和80%生理盐水）。于制模前2 h、制模后1、4、7、10、14 d、PRF治疗后1、4、7、10、14、21、28、35和42 d测定大鼠机械缩足阈值(MWT)和热缩足潜伏期(TWL)。治疗后第42天处死大鼠,取L4-6脊髓组织,采用Western blot法检测脊髓组织微管相关蛋白轻链Lc3Ⅱ和Lc3Ⅰ、Beclin-1和P62蛋白含量,计算Lc3Ⅱ和Lc3Ⅰ比值。RT-qPCR检测Lc3 mRNA和Beclin-l mRNA的表达量。
结果 与Blank组比较,PHN组、Sham组及PRF组RTX处理后大鼠MWT明显降低,TWL明显延长,脊髓组织Lc3Ⅱ/Lc3Ⅰ蛋白含量比值和Beclin-1蛋白含量明显升高,P62蛋白含量明显降低，Lc3 mRNA和Beclin-1 mRNA表达量明显增加(P均<0.05)。与PHN组比较，PRF组大鼠在治疗7 d后MWT明显升高，TWL明显缩短，Lc3Ⅱ/Lc3Ⅰ蛋白含量比值明显降低，Beclin-1蛋白含量明显降低，P62蛋白含量明显增加，Lc3 mRNA和Beclin-1 mRNA表达量明显降低（P均<0.05）。
结论 脉冲射频可能通过抑制脊髓背角组织自噬活性,改善线粒体功能,从而对PHN模型大鼠产生镇痛作用。 |
| 英文摘要: |
Ojective To observe the analgesic effect of pulsed radiofrequency (PRF) on postherpetic neuralgia (PHN) model rats, and to explore the role of autophagy activity in spinal dorsal horn.
Methods Sixty male SD rats were randomly divided into blank control group (group blank), solvent control group (group solvent), model control group (group PHN), sham treatment group (group sham) and treatment group (group PRF), with 12 rats in each group. PHN model was prepared by intraperitoneal injection of resinifratoxin (RTX) 0.2 g/kg in groups PHN, sham and PRF. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured at 2 hours before and 1, 4, 7, 10 and 14 days after modeling, and 1, 4, 7, 10, 14, 21, 28, 35 and 42 d days after PRF treatment. On the 42th day after treatment, five groups of rats were sacrificed and L4-6 spinal cord tissues were taken. The expression of light chain Lc3 II and Lc3 I, Beclin-1 and P62 in spinal cord tissue was detected by Western blot, and the ratio of Lc3 II to Lc3 I was calculated. The expression of Lc3 mRNA, Beclin-l mRNA was detected by RT-qPCR, and the ultrastructure of spinal dorsal horn tissue was observed under transmission electron microscopy.
Results Compared with group blank, the MWT was decreased and the TWL was increased significantly, the ratio of Lc3 II/Lc3 I and the levels of Beclin-1 were increased, whereas the level of P62 protein was decreased significantly after RTX treatment in groups PHN, Sham and PRF (all P < 0.05). Meanwhile, the expressions of Lc3 mRNA and Beclin-1 mRNA were significantly higher in groups PHN, Sham and PRF than in group blank (all P < 0.05). Compared with group PHN, the MWT was increased and the TWL was decreased significantly, the ratio of Lc3 II/Lc3 I and the levels of Beclin-1 were decreased, and the level of P62 protein was increased significantly 7 d later, whereas the expressions of Lc3 mRNA and Beclin-1 mRNA were decreased significantly in group PRF (all P < 0.05).
Conclusion PRF can improve the function of mitochondria by inhibiting the autophagy activity of spinal dorsal horn tissue, thus exerting an analgesic effect on PHN model rats. |
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