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| 吸入高浓度氢气对大鼠创伤性脑损伤的影响 |
| Effects of inhaling high concentration hydrogen on traumatic brain injury in rats |
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| DOI：10.12089/jca.2019.10.018 |
| 中文关键词: 氢气 创伤性脑损伤 凋亡 氧化应激 |
| 英文关键词: Hydrogen gas Traumatic brain injury Apoptosis Oxidative stress |
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| 中文摘要: |
目的 研究高浓度氢气对大鼠创伤性脑损伤的影响及其机制。
方法 雄性SD大鼠30只,体重220～240 g,随机分为三组,每组10只:假手术组(S组)、脑创伤组(T组)和氢气治疗组(H组)。S组暴露硬脑膜但不进行撞击,T组与H组通过液压颅脑损伤撞击建立大鼠创伤性脑损伤模型,H组于术后第1天起每天吸入1 h高浓度氢气(42％H2-21％O2-37％N2),连续7 d。术后48 h采用HE染色法观察脑组织病理改变，免疫组化法检测创伤周围皮质氧化应激相关蛋白MPO和HO-1含量,尼氏染色法观察尼氏小体来判断神经元状态,术后7 d采用Western blot法检测凋亡相关蛋白bcl-2、bax以及caspase-3含量。
结果 与T组比较,H组神经元损伤减轻（P＜0.05）,异常神经细胞数量减少（P＜0.05）,促凋亡蛋白bax、caspase-3及氧化应激相关蛋白MPO、HO-1含量明显降低(P＜0.05),抗凋亡蛋白bcl-2含量明显升高(P＜0.05)。
结论 高浓度氢气可减轻大鼠创伤性脑损伤,其机制可能与氢气抗凋亡以及抗氧化应激作用相关。 |
| 英文摘要: |
Ojective To investigate the effect and mechanism of high concentration hydrogen on traumatic brain injury in rats.
Methods Thirty male Sprague Dawley (SD) rats, weighing 220-240 g, were randomly divided into sham operation group (group S), traumatic brain injury group (group T) and high concentration hydrogen gas treatment group (group H), 10 rats in each group. Rats in group S were exposed dura mater but not impacted. Rats in group T and group H were induced by fluid percussion to establish traumatic brain injury model. Rats in group H inhaled high concentration hydrogen (42％ H2-21％ O2-37％ N2) for 1 h per day continuously for 7 d from the first day of the operation. The pathological changes of brain tissue were observed within 48 h. The expressions of oxidative stress-related proteins MPO and HO-1 were detected by immunohistochemistry. Neuron status was determined by observing Nissl bodies by Nissl staining within 7 d. The expressions of apoptosis-related proteins bcl-2, bax and caspase-3 were detected by Western blot.
Results Compared with group T, the neuronal damage in group H was alleviated （P ＜ 0.05）, the number of abnormal nerve cells was decreased （P ＜ 0.05）, and the expressions of the proapoptotic proteins bax, caspase-3 and oxidative stress-related proteins MPO and HO-1 were significantly decreased (P ＜ 0.05). Compared with group T, the expression of protein bcl-2 was significantly increased in group H(P ＜ 0.05).
Conclusion High concentration of hydrogen gas can alleviate traumatic brain injury in rats, and the mechanism may be related to the anti-apoptosis and anti-oxidative stress effects of hydrogen. |
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