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| 去铁胺对高氧机械通气大鼠肺表面活性蛋白D和抗氧化酶的影响 |
| Effect of deferoxamine on pulmonary surfactant protein D and antioxidant enzymes in rats exposed to hyperoxia ventilation |
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| DOI：10.12089/jca.2019.10.016 |
| 中文关键词: 去铁胺 肺表面活性蛋白D 高氧 机械通气 肺损伤 |
| 英文关键词: Deferoxamine Pulmonary surfactant protein D Hyperoxia Ventilation Lung injury |
| 基金项目: |
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| 中文摘要: |
目的 研究去铁胺(DFO)对高氧机械通气大鼠肺表面活性蛋白D(SP-D)和抗氧化酶的影响。
方法 雄性SD大鼠24只,体重180～220 g。随机分为三组:空气组(M组)、高氧组(H组)和DFO组(D组),每组8只。麻醉状态下,M组行空气(FiO2=21％)机械通气,H组和D组行高氧(FiO2=90％)机械通气。M组和H组尾静脉输注生理盐水1 ml／h，D组尾静脉输注等容量DFO(200 mg／kg,1 ml／h)。机械通气4 h后取左肺制备支气管肺泡灌洗液(BALF),取右肺组织制备肺组织匀浆、计算湿干重比(W／D)并制备病理切片、行病理损伤评分。采用ELISA法测定BALF和肺组织匀浆中SP-D含量、谷胱甘肽还原酶(GR)和黄嘌呤氧化酶(XOD)活性。
结果 H组和D组BALF和肺组织匀浆中SP-D含量明显低于M组(P＜0.05),GR活性明显弱于M组(P＜0.05)，XOD活性明显强于M组(P＜0.05),W／D和病理损伤评分明显高于M组(P＜0.05)。D组SP-D含量明显高于H组(P＜0.05),GR活性明显强于H组(P＜0.05),XOD活性明显弱于H组（P＜0.05），W／D和病理损伤评分明显低于H组(P＜0.05)。
结论 高氧机械通气4 h可造成明显的肺损伤和肺组织SP-D含量降低,去铁胺可增加SP-D含量、减轻肺损伤,其机制与增强肺组织GR活性和降低XOD活性有关。 |
| 英文摘要: |
Ojective To investigate the effect of deferoxamine (DFO) on pulmonary surfactant protein D (SP-D) and antioxidant enzymes in rats exposed to hyperoxia ventilation.
Methods Twenty-four male sprague dawley rats, weighing 180-220 g, were randomly divided into 3 groups (n = 8): air group (group M), hyperoxia group (group H) and DFO group (group D). The rats in group M were ventilated with air (FiO2 = 21％) and the rats in groups H and D were ventilated with oxygen (FiO2 = 90％) for 4 h. Meanwhile, normal saline 1 ml／h was infused via tail vein in groups M and H. The rats in group D were infused intravenously with the same volume of DFO (200 mg／kg, 1 ml／h). Bronchoalveolar lavage fluid (BALF) was prepared in left lung after 4 h of ventilation. The right lung was taken to make tissue homogenate, the wet／dry weight (W／D) ratio was measured, the pathological changes was observed and the injury level was evaluated. The content of pulmonary surfactant protein D (SP-D), the activity of glutathione reductase (GR) and xanthine oxidase (XOD) were detected in BALF and lung homogenate by ELISA.
Results The content of SP-D and the activity of GR in groups H and D were much lower than those in group M (P ＜ 0.05). The activity of XOD, lung W／D and pathological evaluation in groups H and D were much higher than those in group M (P ＜ 0.05). The content of SP-D and the activity of GR in group D were higher than those in group H (P ＜ 0.05). Meanwhile, the activity of XOD, lung W／D and pathological evaluation in group D were significantly lower than those in group H (P ＜ 0.05).
Conclusion Four hours of hyperoxia ventilation causes significant lung injury and SP-D content reduction in lung tissue. Deferoxamine infusion has an effect of increasing SP-D content and alleviating lung injury, and the mechanism is related to the increased GR and decreased XOD activity. |
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