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| 七氟醚暴露致新生大鼠未成熟脑损伤的研究 |
| Effect and mechanism research of sevoflurane exposure on immature brain injury in neonatal rats |
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| DOI：10.12089/jca.2019.05.021 |
| 中文关键词: 七氟醚 未成熟脑 星型胶质细胞 新生大鼠 Morris水迷宫 |
| 英文关键词: Sevoflurane Immature brain Astrocytes Neonatal rat Morris water maze |
| 基金项目:十堰市太和医院院级项目（2017JJXM075） |
| 作者 | 单位 | E-mail | | 刘光建 | 442000,湖北省十堰市太和医院麻醉科,湖北医药学院附属太和医院,湖北医药学院麻醉学研究所实验室 | | | 魏琼 | 442000,湖北省十堰市太和医院麻醉科,湖北医药学院附属太和医院,湖北医药学院麻醉学研究所实验室 | | | 李清 | 442000,湖北省十堰市太和医院麻醉科,湖北医药学院附属太和医院,湖北医药学院麻醉学研究所实验室 | | | 罗向红 | 442000,湖北省十堰市太和医院麻醉科,湖北医药学院附属太和医院,湖北医药学院麻醉学研究所实验室 | 95luoxianghong@163.com |
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| 中文摘要: |
目的 探讨七氟醚早期暴露对新生大鼠脑发育的影响。
方法 选取新生6日龄SD大鼠80只，随机分为四组，每组20只，分别为空白对照组(C组)，1%七氟醚暴露2 h组（Sev1组），2%七氟醚暴露2 h组（Sev2组），4%七氟醚暴露2 h组（Sev3组）；七氟醚暴露组分别置于1%、2%、4%七氟醚中每天暴露2 h，连续3 d；出生后第9天采用0.5%戊巴比妥钠麻醉后处死新生大鼠取出脑组织；采用电子天平秤检测各组大鼠在出生后6、9、15、28 d体重和脑重的变化；采用荧光定量PCR法（RT-PCR）检测Cx30、IL-6及BDNF基因mRNA表达量；采用免疫荧光法检测GFP蛋白含量；出生后28 d Morris水迷宫评价新生大鼠学习记忆能力。
结果 与C组比较，Sev1、Sev2和Sev3组新生大鼠体质量和脑质量均有所减轻，且随着日期延长这种减轻更为明显，Cx30 mRNA和BDNF mRNA表达量明显降低，IL-6mRNA表达量明显升高（P<0.05），GFP细胞数量明显减少（P<0.05）；与Sev1组比较，Sev2和Sev3组新生大鼠体质量和脑质量均明显减轻，GFP数量明显减少（P<0.05）；与C组比较，Sev1、Sev2和Sev3组逃避潜伏期和游动距离均明显延长（P<0.05）,目标象限停留时间明显缩短（P<0.05），穿越虚拟平台次数明显减少（P<0.05）。
结论 新生大鼠接受七氟醚暴露可致未成熟脑损伤及远期学习记忆能力下降，且这种脑损伤程度与七氟醚浓度呈正相关；其机制可能与其脑内Cx30、BDNF基因表达水平改变有关。 |
| 英文摘要: |
Ojective To investigate the effect and mechanism of sevoflurane exposure on immature brain injury in neonatal rats.
Methods Eighty 6-day-SD neonatal rats were randomly divided into four groups, twenty rats in each group: blank control group(group C), 1% sevoflurane 2 h-exposure group(group sev1), and 2% sevoflurane 2 h-exposure group(group sev2), 4% sevoflurane 2 h-exposure group(group Sev3). Sevoflurane exposure group were placed in 1%, 2%, 4% sevoflurane exposure 2h daily for 3 days respectivelly. The neonatal rats were sacrificed after anesthesia with 0.5% pentobarbital sodium and brain tissues were removed until the 9th day after birth. The body weights and brain weights were measured on the 6th, 9th, 15th, and 28th day after birth. The expression of Cx30, IL-6 and BDNF mRNA were identified by fluorescence quantitative PCR (RT-PCR). The expressions of GFP protein were detected by immunofluorescence. The ability of memorize and study in these rats were assessed using Morris water maze postnatal day 28.
Results Compared with group C, the body weight and brain mass of the neonatal rats in groups Sev1, Sev2 and Sev3 were decreased, and the reduction was more pronounced with the extension of the date; the expression of Cx30 mRNA and BDNF mRNA was down-regulated, and the expression of IL-6 mRNA was up-regulated significantly (P < 0.05); the number of GFP+ cells was decreased significantly (P < 0.05). Compared with group Sev1, body weight and brain mass of newborn rats in groups Sev2 and Sev3 also decreased, and the number of GFP+ cells was decreased significantly (P < 0.05). Compared with group C, the time of escape latency of groups Sev1, Sev2 and Sev3 after trains was significantly longer (P < 0.05); the time in target quadrant were significantly shorter (P<0.05); the number of times through virtual platform were significantly lower (P < 0.05).
Conclusion Exposure of sevoflurane could result in immature brain development disorders and long-term learning and memory deficits, and the extent of brain damage is positively correlated to the concentration of sevoflurane; the mechanism may be related to the changes of intracerebral Cx30 and BDNF expression. |
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