|
| 丙泊酚对大鼠离体海马CA1区场电位的作用 |
| Effect of propofol on the neuronal oscillations in CA1 area of rat intact hippocampus |
| |
| DOI：10.12089/jca.2019.05.019 |
| 中文关键词: 丙泊酚 离体完整海马 场电位 γ-氨基丁酸A型受体 |
| 英文关键词: Propofol Hippocampus Local field potential γ-aminobutyric acid type A receptor |
| 基金项目:国家自然科学基金重点项目（81730031） |
|
| 摘要点击次数: 2688 |
| 全文下载次数: 970 |
| 中文摘要: |
目的 观察临床常用的静脉麻醉药丙泊酚对大鼠离体完整海马CA1区场电位不同振荡频段的作用，探讨丙泊酚对海马内部神经环路功能可能产生的影响。
方法 选择出生后14~16 d雄性SD大鼠于4℃条件下进行完整海马的剥离。待孵育1~2 h稳定后，移至电生理记录槽。实验根据灌流的丙泊酚浓度不同分为对照组（C组）、丙泊酚1 μmol/L组（P1组）、丙泊酚20 μmol/L组（P20组）、丙泊酚40 μmol/L组（P40组）和丙泊酚80 μmol/L组（P80组）。C组不予丙泊酚灌流，为基础值，P1组、P20组、P40组和P80组分别给予丙泊酚1、20、40和80 μmol/L灌流，采用玻璃微电极记录离体完整海马CA1区辐射层的场电位信号，进行场电位的功率谱强度分析。将C组的基础值设定为1，分别计算并比较P1组、P20组、P40组和P80组离体完整海马CA1区场电位不同振荡频段（δ：1~4 Hz,θ：4~8 Hz，α：8~13 Hz，β：13~30 Hz，γ：30~80 Hz）与C组比值。
结果 与C组比较，P1组场电位θ频段明显增强（P<0.05），γ频段明显减弱（P<0.05），P20组、P40组和P80组场电位不同振荡频段均明显减弱（P<0.05）。与P1组比较，P20组场电位δ、θ、α、β频段明显减弱（P<0.05），P40组和P80组场电位不同振荡频段均明显减弱（P<0.05）。与P20组比较，P80组场电位δ、θ、α频段明显减弱（P<0.05）。与P40组比较，P80组场电位δ频段明显减弱（P<0.05）。
结论 除θ频段外，丙泊酚对海马CA1区场电位的δ、α、β和γ频段呈现浓度依赖的抑制作用；对于θ频段，大于20 μmol/L浓度的丙泊酚仍然表现为抑制作用，但在1 μmol/L浓度下却表现为增强作用，显示低浓度丙泊酚对海马内部神经环路可能存在兴奋作用。 |
| 英文摘要: |
Ojective To reveal the effect of propofol on hippocampal neuroal circuits, local field potential (LFP) in CA1 region of intact hippocampus in vitro.
Methods SD rats aged 14 - 16 days postnatal were anesthetized and decapitated. The hippocampal formation was dissected from brain tissue in 4℃. LFP of hippocampal CA1 stratum radiatum was recorded with glass electrode when the bath was perfused with propofol at 5 different concentrations-control group with no propofol (group C), 1 μmol/L (group P1), 20 μmol/L(group P20), 40 μmol/L (group P40) and 80 μmol/L (group P80), respectively. The effect of propofol on the LFP power spectrum (δ: 1~4 Hz, θ: 4~8 Hz, α: 8~13 Hz, β: 13~30 Hz, γ: 30~80 Hz) in CA1 region of intact hippocampus was analyzed.
Results Compared with group C, θ oscillation in group P1 was significantly increased （P < 0.05）, γ oscillation was significantly decreased （P < 0.05）, while all oscillations of LFP power spectrum were obviously decreased in groups P20, P40 and P80（P < 0.05）. Compared with group P1, δ, θ, α and β oscillations in group P20 were significantly decreased （P < 0.05）. And all oscillations of LFP power spectrum in groups P40 and P80 were significantly decreased （P < 0.05）. Compared with group P20, δ、θ、α oscillations in groups P40 were significantly decreased （P < 0.05）. Compared with group P40, δ oscillation in group P80 was significantly decreased （P < 0.05）.
Conclusion Propofol has a concentration-dependent inhibitory effect on δ, α, β and γ oscillations of hippocampal CA1. Although propofol at over 20 μmol/L inhibits θ oscillation, propofol at 1 μmol/L enhances θ oscillation, suggesting that propofol at low concentration exhibits exciting effects on the hippocampal internal neural circuits. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
