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| 七氟醚麻醉对大鼠海马α7烟碱型乙酰胆碱受体、胆碱酯酶和胆碱乙酰移位酶蛋白含量的影响 |
| Effects of sevoflurane anesthesia on the quantity of alpha 7 nicotinic acetylcholine receptor, cholinesterase and choline acetyl translocase in the rat hippocampus |
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| DOI：10.12089/jca.2019.02.014 |
| 中文关键词: 七氟醚 α7烟碱型乙酰胆碱受体 乙酰胆碱酯酶 胆碱乙酰移位酶 认知功能 |
| 英文关键词: Sevoflurane Alpha 7 nicotinic acetylcholine receptor Cholinesterase Choline acetyl translocase Cognitive function |
| 基金项目:国家自然科学基金（81360180，81660193） |
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| 摘要点击次数: 3749 |
| 全文下载次数: 938 |
| 中文摘要: |
目的 研究七氟醚麻醉对大鼠海马α7烟碱型乙酰胆碱受体（α7nAchR）、胆碱酯酶（AChE）、胆碱乙酰移位酶（ChaT）蛋白含量的影响。
方法 健康SD新生大鼠120只，雌雄不拘，1周龄，随机分为五组：空白组、空氧组、七氟醚组（SEV组）、α7nAchR激动剂组（PNU组）和α7nAchR拮抗剂组(MLA组)，每组24只。空白组自由喂养;空氧组吸入60%的氧气即运载气体（1 L/min O2+1 L/min空气）2 h;SEV组吸入3.4%七氟醚+运载气体2 h;PNU组单次腹腔注射α7nAchR激动剂PNU-282987，24 h后吸入3.4%七氟醚+运载气体2 h;MLA组单次腹腔注射α7nAchR拮抗剂甲基牛扁亭，24 h后吸入3.4%七氟醚+运载气体2 h。麻醉清醒后2 h、1周、4周取海马组织，采用Western blot法测定α7nAchR、AChE、ChaT蛋白含量。
结果 麻醉清醒后2 h SEV组、PNU组、MLA组α7nAchR蛋白含量明显低于空氧组（P<0.05）;PNU组、MLA组AChE蛋白含量明显高于空氧组（P<0.05）;SEV组、PNU组、MLA组ChaT蛋白含量明显低于空氧组（P<0.05）。麻醉清醒后1周空白组、SEV组、PNU组海马中α7nAchR蛋白含量明显高于空氧组（P<0.05），MLA组α7nAchR蛋白含量明显低于空氧组（P<0.05）;空白组、PNU组AChE蛋白含量明显高于空氧组（P<0.05）;空白组ChaT蛋白含量明显高于空氧组，SEV组ChaT蛋白含量明显低于空氧组（P<0.05）。麻醉清醒后4周五组AChE蛋白含量差异无统计学意义;SEV组α7nAchR蛋白含量明显高于空白组，PUN组和MLA组明显低于空白组（P<0.05）;空白组、SEV组和PNU组ChaT蛋白含量明显低于空氧组，MLA组明显高于空氧组（P<0.05）。
结论 吸入七氟醚后能抑制ChaT、α7nAChR;对AChE并无直接作用;α7nAChR激动剂能有效缓解吸入七氟醚对α7nAChR、ChaT抑制作用，并在1周左右达到高峰;氧浓度在60%左右能增加α7nAChR蛋白含量，一定程度对抗七氟醚抑制作用。 |
| 英文摘要: |
Ojective To investigate the expression of alpha 7 nicotinic acetylcholine receptor (α7nAchR), cholinesterase (AChE), choline acetyl translocase (ChaT) after sevoflurane anesthesia.
Methods A total of 120 healthy Sprague-Dawley rats with both two genders, aged 1 week, were randomly divided into 5 groups: blank group; air/O2 group; sevoflurane group (group SEV); α7nAchR agonist group (group PUN); α7nAchR antagonist group (group MLA), 24 in each group. Blank group received free feeding, air/O2 group was inhaled 60% oxygen (carrier gas: 1 L/min O2+1 L/min air) 2 h; group SEV was inhaled 3.4% sevoflurane and carrier gas for 2 h; group PUN and group MLA were injected with PNU-282987 and methyllycaconitine, respectively, after 24 h inhaled of 3.4% sevoflurane and carrier gas for 2 h. After that, hippocampus dissection carried out in 2 h, 1 w, 4 w, and Western blot method was used to detect α7nAchR, AChE, ChaT proteins expression.
Results Two hours after anesthesia recovery, α7nAchR in groups SEV, PNU and MLA was significantly lower than that in air/O2 group (P < 0.05); AChE in groups PNU and MLA was significantly lower than that in air/O2 group (P < 0.05); ChaT in groups SEV, PNU and MLA was significantly lower than that in air/O2 group (P < 0.05). One week after anesthesia recovery, α7nAchR in blank group and groups SEV and PNU was significantly higher than that in air/O2 group (P < 0.05), α7nAchR in group MLA was significantly lower than that in air/O2 group (P < 0.05); AChE in blank group and and group PNU was significantly higher than that in air/O2 group (P < 0.05), ChaT in blank group was significantly higher than that in air/O2 group (P < 0.05), ChaT in group SEV was significantly lower than that in air/O2 group (P < 0.05). Four weeks after anesthesia awake, AChE in each group was not statistically significant; α7nAchR in group SEV was significantly higher than that in blank group (P < 0.05), α7nAchR in group PNU and MLA was significantly lower than that in blank group (P < 0.05); ChaT in blank group and group PNU was significantly lower than that in air/O2 group (P < 0.05), ChaT in group MLA was significantly higher than that in air/O2 group (P < 0.05).
Conclusion Sevoflurane inhalation can inhibit ChaT, α7nAChR， which had no direct effect on AChE; α7nAChR agonist can effectively help α7nAChR and ChaT inhibition inhaled sevoflurane, and reached a peak at about 1 week; oxygen concentration around 60% can increase α7nAChR expression quantity, to a certain extent against sevoflurane inhibition. |
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