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| 脓毒症相关性脑病大鼠静息态神经网络功能改变 |
| Abnormality of resting-state functional network in a rat animal model of sepsis-associated encephalopathy |
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| DOI：10.12089/jca.2019.01.013 |
| 中文关键词: 脓毒症 情感 功能连接 神经网络 |
| 英文关键词: Sepsis Mood Functional connectivity Neural circuit |
| 基金项目:国家自然科学基金(81771156,81772126) |
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| 摘要点击次数: 3799 |
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| 中文摘要: |
目的 采用静息态功能磁共振(rs-fMRI)观察脓毒症相关性脑病(SAE)大鼠神经网络功能改变。
方法 健康雄性SD大鼠30只,随机分为对照组和SAE组。SAE组大鼠采用腹腔注射脂多糖(LPS) 1 mg/kg建立SAE模型，对照组大鼠腹腔注射等容量生理盐水。使用区域一致性(ReHo)方法检测大鼠异常脑区并将其作为种子点进行全脑功能连接分析，并在LPS腹腔注射48 h后进行行为学测试。
结果 与对照组比较,SAE组在强迫游泳实验不动时间明显延长[(38.93±13.84) s vs (22.06±6.75) s，P<0.05];前扣带回皮质 (ACC)及右侧尾状核(CPu)ReHo值明显增加,分别为(1.21±0.07 vs 0.97±0.12,P<0.05)及(1.34±0.09 vs 1.17±0.16,P<0.05);将ACC作为种子点,其与后扣带回皮质(RSC)的功能连接明显增强(0.45±0.06 vs 0.11±0.02,P<0.05);将右侧CPu作为种子点,其与左侧CPu的功能连接明显增(0.33±0.07 vs 0.07±0.01,P<0.05);抑郁样行为与ACC(r2=0.357 3,P=0.018 6)及右侧CPu(r2=0.5036,P=0.003 0)升高的ReHo值以及双侧CPu之间增强的功能连接(r2=0.315 9,P=0.029 2)呈现一定的相关性。
结论 ACC和右侧CPu ReHo值的增高及双侧CPu功能连接的增强可能参与大鼠SAE的情感损害。 |
| 英文摘要: |
Ojective To explore changes in the functional network in an animal model of SAE induced by lipopolysaccharide (LPS) challenge.
Methods Thirty male Sprague-Dawley rats were randomly allocated into control group and group SAE. SAE model was established by intraperitoneal injection of LPS 1 mg/kg in group SAE , while the same amount of saline. was injected in the control group. Firstly, we used regional homogeneity (ReHo) method to exam abnormal brain regions between the control and SAE groups and then considered them as seeds for functional connectivity analysis, and behavioral tests were performed at 48 hours after LPS intraperitoneal injection.
Results Compared with the control group, our study showed LPS impaired mood function, as reflected by depression-like behavior of prolonged immobility [(38.93 ± 13.84)s vs (22.06 ± 6.75)s,P < 0.05] in the forced swim test; LPS induced significantly increased ReHo values in the anterior cingulate cortex (ACC) and caudate putamen (CPu) of (1.21 ± 0.07 vs 0.97 ± 0.12, P < 0.05) and (1.34 ± 0.09 vs 1.17 ± 0.16, P < 0.05) respectively; the ACC seed showed increased functional connectivity with the retrosplenial cortex (0.45 ± 0.06 vs 0.11 ± 0.02, P<0.05); the right CPu seed showed increased functional connectivity with the left Cpu (0.33 ± 0.07 vs 0.07±0.01, P<0.05); the depression-like behavior was associated with increased ReHo values in the ACC and right CPu as well as increased functional connectivity between the right CPu and left CPu.
Conclusion This study suggests that the impaired mood function in SAE is associated with increased ReHo values in the ACC and right CPu as well as increased functional connectivity between the right CPu and left CPu, indicating that the abnormality of resting-state functional network may be a potential mechanism involved in SAE. |
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