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| 参麦注射液预处理对老龄大鼠术后认知功能的影响 |
| Effects of Shenmai injection pretreatment on postoperative cognition in old rats |
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| DOI：10.12089/jca.2018.08.015 |
| 中文关键词: 术后认知功能 炎症 参麦注射液 布洛芬 |
| 英文关键词: Postoperative cognition Inflammation Shenmai injection Ibuprofen |
| 基金项目:山东省自然科学基金重点项目（ZR2014HZ005） |
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| 中文摘要: |
目的 观察参麦注射液预处理对老龄大鼠术后炎症反应水平和认知功能的影响。
方法 健康雄性老龄SD大鼠72只,月龄约20个月，体重500～600 g。按随机数字表法分为对照组(C组)、参麦注射液组(S组)和布洛芬组(I组),每组24只。S组腹腔注射参麦注射液,I组给予布洛芬悬液灌胃,C组腹腔注射等量生理盐水。七氟醚麻醉下行脾切除术建立老龄大鼠POCD模型。三组大鼠分别于麻醉后即刻、术后即刻和术后1 d 采集颈静脉血,采用ELISA固相夹心法测定IL-1β、IL-6和TNF-α浓度。于术前、术后1、3和7 d进行 Morris水迷宫实验和旷场实验,记录大鼠的逃避潜伏期、平台穿越次数、中央格停留时间、修饰次数和直立次数。
结果 术后即刻,S组和I组IL-1β、IL-6和TNF-α浓度均明显低于C组(P<0.01或P<0.05);术后1 d,I组IL-1β与S组和I组IL-6的浓度均明显低于C组(P<0.01或P<0.05)。与术前比较,术后1、3 d C组和S组的逃避潜伏期明显延长(P<0.05),平台穿越次数明显减少(P < 0.01或P < 0.05),中央格停留时间明显延长(P<0.01或P<0.05),修饰和直立次数均明显减少(P<0.01或P<0.05)。与C组和S组比较,术后1、3 d I组逃避潜伏期明显缩短(P<0.01或P<0.05),平台穿越次数均明显增多(P<0.01或P<0.05),中央格停留时间明显缩短(P<0.01),修饰次数和直立次数均明显增多(P<0.01或P<0.05)。
结论 参麦注射液预处理可减轻手术后炎症反应,但对POCD无明显影响。 |
| 英文摘要: |
Objective To investigate the effects of shenmai injection (SMI) pretreatment on inflammatory response and postoperative cognition in old rats.
Methods Seventy-two healthy male SD rats, aged 20 months, weighing 500 - 600 g, were randomly divided into three groups (n = 24 each): control group (group C), SMI group (group S) and ibuprofen group (group I). Group C received 2 ml of saline by intraperitoneal injection, group S received 2 ml of SMI by intraperitoneal injection, and group I received 3.5 mg/100 g of ibuprofen suspension. The POCD models of old rats were made by splenectomy under sevoflurane anesthesia. Blood was drawn from the jugular vein immediately after anesthesia, immediately after operation and 1 day after operation. IL-1, IL-6 and TNF-α were measured by ELISA. The learning and memorizing capability of rats were observed through the Morris water maze and Open field test before surgery and at 1, 3 and 7 days after operation.
Results Immediately after operation, the concentrations of IL-1β, IL-6 and TNF-α in group S and group I were significantly lower than those in group C (P < 0.01 or P < 0.05). The concentrations of IL-1β in group I and IL-6 in group S and group I were significantly lower than that in group C on day 1 postoperatively (P < 0.01 or P < 0.05). Compared with before operation, the escape latency of group C and group S was significantly prolonged (P < 0.05), the number of platform crossings was significantly reduced (P < 0.01 or P < 0.05), and the central lattice retention time was significantly prolonged (P < 0.01 or P < 0.05), the number of modification and erection were significantly decreased (P < 0.01 or P < 0.05) on day 1 and day 3 postoperatively, there was no significant change in group I. Compared with group C and group S, the escape latency of group I was significantly shorter (P < 0.01 or P < 0.05), and the number of platform crossings was significantly increased (P < 0.01 or P < 0.05); the central lattice retention time of group I significantly reduced (P < 0.01); the number of modification and erection significantly increased (P < 0.01 or P < 0.05) on day 1 and day 3 postoperatively; there was no significant difference between group S and group C.
Conclusion Shenmai injection pretreatment can reduce postoperative inflammatory response, but no significant effect on postoperative cognitive dysfuncion. |
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