文章摘要
吡格列酮对老年大鼠术后认知功能的影响及其机制研究
Effect and mechanisms of pioglitazone on post operative cognitive function induced by surgery in elderly rats
  
DOI：10.12089/jca.2018.05.016
中文关键词: 吡格列酮  术后认知功能  高级糖基化终末产物  白细胞介素-6  活性氧
英文关键词: Pioglitazone  Postoperative cognitive function  Interleukin-6  Advanced glycation endproducts  Reactive oxygen species
基金项目:中山大学2017年度高校基本业务费（17ykjc15）
作者单位E-mail
刘付宁 510120,广州市,中山大学孙逸仙纪念医院麻醉科  
黄浩泉 510120,广州市,中山大学孙逸仙纪念医院麻醉科  
刘玲 510120,广州市,中山大学孙逸仙纪念医院麻醉科  
郭明炎 510120,广州市,中山大学孙逸仙纪念医院麻醉科  
杨浩杰 510120,广州市,中山大学孙逸仙纪念医院麻醉科  
曹铭辉 510120,广州市,中山大学孙逸仙纪念医院麻醉科 caofox5188@126.com 
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中文摘要:
      目的 探讨吡格列酮对老年大鼠术后认知功能的影响及其机制。方法 18月龄雄性SD大鼠160只，随机为四组: 对照组(Con组)、手术组(S组) 、吡格列酮组(P组)及吡格列酮预处理手术组(PS组)。手术模型完成时测定各组大鼠血糖值;手术结束12 h后,采用Western blot法检测各组大鼠海马组织高级糖基化终末产物(advanced glycation endproducts, AGEs)含量,采用ELISA法检测各组大鼠海马组织活性氧(ROS)及IL-6浓度;术后第4天开始应用Morris水迷宫方法 评价各组大鼠认知功能。结果 四组大鼠组间组内血糖差异无统计学意义。 与Con组比较, 手术结束12 h后S组大鼠海马AGEs、IL-6浓度明显增加, ROS浓度明显升高(P＜0.05);与S组比较, PS组大鼠海马AGEs、IL-6浓度明显减少, ROS浓度明显降低(P＜0.05)。与Con组比较, S组大鼠术后第8天和第10天逃逸潜伏期明显延长(P＜0.05), 测试时穿越平台的次数减少(P＜0.01);与S组比较, PS组大鼠术后第10天逃逸潜伏期明显缩短(P＜0.05), 测试时穿越平台的次数明显增加(P＜0.05)。结论 吡格列酮可能不是直接通过调节大鼠的血糖变化改善老年大鼠术后认知功能,其作用机制可能与减少AGEs合成、降低ROS及IL-6浓度有关。
英文摘要:
      Ojective To investigate the effect and mechanisms of pioglitazone on post operative cognitive function induced by surgery in elderly rats. Methods Elderly SD rats, male and weighing 220-250 g, were randomly divided into control group (group Con), surgery group (group S), pioglitazone group (group P) and pioglitazone/surgery group (group PS). The level of glycemia was measured by glucometer after completing the surgical model. The expression of advanced glycation endproducts (AGEs) in hippocampal tissues was detected by western blot and reactive oxygen species (ROS) and IL-6 by ELISA 12 h after surgery. Morris water maze was used for evaluation of cognitive function 4 days after surgery. Results Compared with group Con, group S showed a significant increase in the expression of AGEs and IL-6 and the level of ROS (P＜0.05); Compared with group S, the expression of AGEs and IL-6 and the level of ROS decreased significantly in group PS (P＜0.05). Compared to group Con, the surgery increased the average time of escape latencies on the 8th days and 10th days after surgery and reduced the platform-crossing times in the Morris water maze test (P＜0.05). Compared with group S, group PS showed a significant decrease in the average time of escaping latencies on the 10th day after surgery (P＜0.05), and an increased platform-crossing times in the Morris water maze test (P＜0.05). Conclusion These results suggest that pioglitazone attenuates postoperative cognitive function and its mechanism may be related to the decrease of the expression of AGEs and IL-6 and the level of ROS.
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