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| Fas/FasL在罗哌卡因致PC12细胞凋亡中的表达 |
| Expression of Fas/FasL in PC12 cell apoptosis induced by ropivacaine |
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| DOI：10.12089/jca.2018.04.014 |
| 中文关键词: 罗哌卡因 PC12细胞 细胞凋亡 Fas FasL |
| 英文关键词: Ropivacaine PC12 cells Apoptosis Fas FasL |
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| 中文摘要: |
| 目的 检测Fas、FasL在罗哌卡因诱导PC12细胞凋亡中表达的变化,探讨罗哌卡因的神经毒性机制。方法 采用不同浓度罗哌卡因(0.1、0.5、1、2、4 mmol/L)处理PC12细胞24 h以建立细胞的神经毒性模型,CCK-8法测定细胞活力。最终将细胞随机分为三组:0.5 mmol/L组、2 mmol/L组和正常对照组。各组细胞培养24 h后用光学显微镜观察细胞形态学变化(加上1 mmol/L组),流式细胞仪检测细胞凋亡,免疫荧光检测Fas、FasL表达。结果 与正常对照组比较,0.5 mmol/L组和2 mmol/L组细胞活力明显降低(P＜0.05),细胞形态明显异常(包括1 mmol/L组),凋亡率明显升高(P＜0.05),Fas、FasL表达明显增强(P＜0.05);与0.5 mmol/L组比较,2 mmol/L组细胞凋亡率和Fas、FasL表达明显增加(P＜0.05)。结论 罗哌卡因可诱导PC12细胞凋亡,其机制可能与Fas/FasL上调有关。 |
| 英文摘要: |
| Objective To detect the expression of Fas and FasL in ropivacaine-induced rat phechromocytoma (PC12) cells apoptosis and the mechanism of its neurotoxicity. Methods PC12 cells were treated with different concentrations of ropivacaine (0.1, 0.5, 1, 2, and 4 mmol/L) for 24 h to establish a cellular neurotoxicity model, and the cell viability were assessed by CCK-8. The cells were finally divided into 3 groups randomly: 0.5 mmol/L group, 2 mmol/L group and control group. After the cells were cultured for 24 h, morphological changes of cells were observed under optical microscope (add the 1 mmol/L group), apoptosis was assessed by flow cytometer,the expression of Fas and FasL were assessed by immunofluorescence. Results Compared with the control group,the cell viability of 0.5 mmol/L and 2 mmol/L group decreased significantly (P＜0.05), the cells exhibited obvious morphologic abnormalities (including the 1 mmol/L group), the apoptotic rate increased significantly (P＜0.05), the expression of Fas and FasL increased significantly (P＜0.05); Compared with 0.5 mmol/L group,the apoptotic rate and expression of Fas, FasL of 2 mmol/L group increased significantly (P＜0.05). Conclusion Ropivacaine explosure induces apoptosis in PC12 cells, which might be related with the up-regulation of Fas/FasL. |
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