文章摘要
麻醉期间低体温对新生大鼠学习记忆能力的影响
Effects of hypothermia on learning and memory ability of neonatal rats during anesthesia
  
DOI：10.12089/jca.2018.03.018
中文关键词: 低体温  细胞外信号调节激酶  环磷酸腺苷应答元件结合蛋白  新生大鼠  海马
英文关键词: Hypothermia  Extracellular signal regulated kinase  Cyclic AMP response element binding protein  Neonatal rats  Hippocampus
基金项目:贵州省科技计划项目(黔科合LH字［2017］7202号)
作者单位E-mail
刘文博 550004,贵阳市,贵州医科大学麻醉学院  
杨剑 550004,贵阳市,贵州医科大学麻醉学院 18786085605@139.com 
熊兴龙 550004,贵阳市,贵州医科大学麻醉学院  
高鸿 550004,贵阳市,贵州医科大学麻醉学院  
刘婉玲 550004,贵阳市,贵州医科大学麻醉学院  
王礼鑫 550004,贵阳市,贵州医科大学病理教研室(王礼鑫)  
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中文摘要:
      目的 探讨麻醉期间低体温状态对新生大鼠学习记忆能力的影响及其可能的作用机制。方法 出生7 d的SD新生大鼠40只,按随机数字表法分为四组(n=10):对照组(C组)、麻醉保温组(A组)、麻醉低温组(AH组)、物理低温组(H组)。C组腹腔注射0.1 ml生理盐水并配备加热垫保持直肠温度在38～39℃;A组以25 mg/kg腹腔注射丙泊酚0.1 ml,每20分钟追加初始剂量的1/2,麻醉维持2 h,同C组方法 保持直肠温度在38～39℃;AH组麻醉方式和时间与A组相同,大鼠在麻醉过程中不保温,控制室温为23℃,允许体温自然下降;H组大鼠腹腔注射0.1 ml生理盐水,同样控制室温为23℃,使体温自然下降。麻醉过程中持续吸氧,2 h内每20分钟监测记录一次体温。清醒后即刻,每组随机取5只大鼠采用Western blot法检测海马磷酸化-细胞外信号调节激酶(p-ERK)、磷酸化-环磷酸腺苷应答元件结合蛋白(p-CREB)相对表达量。每组剩余5 只大鼠继续饲养至30日龄进行水迷宫实验检测其空间学习记忆能力和海马p-ERK、p-CREB相对表达量。结果 降温60 min,AH、H组大鼠的直肠温度分别下降至(25.38±0.22)℃、(25.54±0.20)℃,明显低于C组(38.36±0.24)℃和A组(37.40±0.29)℃(P＜0.05)。清醒后即刻,AH、H组p-ERK和p-CREB蛋白相对表达量明显低于C组和A组(P＜0.05)。30 d四组大鼠逃避潜伏期、穿越平台次数和原平台象限逗留时间差异无统计学意义。水迷宫测试结束时,四组大鼠p-ERK和p-CREB蛋白相对表达量差异无统计学意义。结论 麻醉期间体温降至25℃时可短期抑制新生大鼠海马p-ERK、p-CREB表达,而对其远期学习记忆能力无明显影响。
英文摘要:
      Objective To investigate the effect of hypothermia on the learning and memory ability of neonatal rats during anesthesia and its possible mechanism. Methods Forty SD rats aged 7 d were randomly divided into 4 groups (n=10): control group (group C), anesthesia group (group A), anesthesia and hypothermia group (group AH)， hypothermia group (group H). Group C was injected intraperitoneally with 0.1 ml of saline and equipped with heating pad to keep the rectal temperature at 38-39℃. Group A was injected with propofol 0.1 ml at 25 mg/kg intraperitoneally with 1/2 of the initial dose, and anesthesia was maintained for 2 h. The same method to maintain the rectal temperature at 38-39℃; Group AH of anesthesia and time and Group A of the same, rats in the anesthesia process is not insulation, control room temperature of 23℃, allowing the body temperature decreased; Group H rats intraperitoneal injection of 0.1 ml saline, the same control room temperature of 23℃, so that the natural temperature drop. Anesthesia in the process of continuous oxygen, intermittent monitoring of body temperature. Immediately after awake, 5 rats in each group were randomly selected to detect the content of p-ERK and p-CREB in hippocampus by Western blot. The spatial learning and memory ability and p-ERK and p-CREB contents in hippocampus were measured by water maze test. Results The rectal temperature in group AH and group H decreased to (25.38±0.22)℃ and (25.54±0.20)℃ in 1 h respectively, and the body temperature decreased significantly compared with group C (38.36±0.24)℃ and group A (37.40±0.29)℃ (P＜0.05). Compared with group C and group A, the expression of group AH and group H was decreased (P＜0.05). At the end of the water maze test, the expression of protein in the four groups was not statistically significant. There was no significant difference between the four groups in the escape latency, the number of crossing the platform and the quadrant of the original platform quadrant. Conclusion The expression of p-ERK and p-CREB in neonatal rats hippocampus can be short-term inhibition in the period of hypothermia at 25℃, but no significant effect on the long-term learning and memory ability.
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