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| 七氟醚后处理对糖尿病大鼠脑缺血-再灌注损伤的影响 |
| Effects of sevoflurane post treatment on cerebral ischemia-reperfusion injury in diabetic rats and non-diabetic rats |
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| DOI：10.12089/jca.2018.02.017 |
| 中文关键词: 七氟醚 糖尿病 缺血-再灌注损伤 血管生成素-1 |
| 英文关键词: Sevoflurane Diabetes Ischemia-reperfusion injury Angiogenin-1 |
| 基金项目:潍坊市科学技术发展计划项目（2015WS084） |
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| 中文摘要: |
| 目的 研究七氟醚后处理对糖尿病大鼠脑缺血-再灌注损伤的影响。方法 雄性SD大鼠32只, 3.0～3.5月龄, 体重280～320 g, 采用随机数字表法分为四组: 非糖尿病缺血-再灌注对照组(NDC组)、糖尿病缺血-再灌注对照组(DC组)、非糖尿病缺血-再灌注七氟醚后处理组(NDS组)和糖尿病缺血-再灌注七氟醚后处理组(DS组), 每组8只。采用栓线法和链脲佐霉素制备缺血-再灌注损伤大鼠模型和糖尿病大鼠模型。缺血2 h, 再灌注24 h后, 进行大鼠神经缺损评分, 采用TTC法测定脑梗死体积, Western blot法测定血管生成素-1(angiopoiettin-1, Ang-1)蛋白含量。结果 DC组神经缺损评分、脑梗死体积百分比明显高于NDC组, Ang-1蛋白含量明显低于NDC组(P＜0.05);NDS组神经缺损评分、脑梗死体积百分比明显低于NDC组, Ang-1蛋白含量明显高于NDC组(P＜0.05);DS组神经缺损评分、脑梗死体积百分比明显低于DC组, Ang-1蛋白含量明显高于DC组(P＜0.05)。结论 大鼠脑缺血-再灌注后, 糖尿病可加重神经缺损, 增大脑梗死体积, 减少Ang-1蛋白含量;七氟醚可减轻神经缺损, 减小脑梗死体积, 增加Ang-1蛋白含量;七氟醚后处理减轻大鼠脑缺血-再灌注损伤与Ang-1蛋白含量增加有关。 |
| 英文摘要: |
| Objective To investigate the effects of sevoflurane post treatment on cerebral ischemia-reperfusion injury in diabetic rats and non-diabetic rats. Methods Thirty-two male SD rats, aged 3.0-3.5 months, weighing 280-320 g, were divided into four groups by random number method (n=8): non diabetic ischemia reperfusion group (group NDC); diabetic ischemia reperfusion group (group DC); non diabetic ischemia reperfusion group with the sevoflurane post-treatment (group NDS); diabetic ischemia reperfusion group with the sevoflurane post-treatment (group DS). The middle cerebral artery occlusion method and streptozotocin were used to establish the ischemia-reperfusion injury model and diabetic model. 2 h after ischemia and 24 h after reperfusion, the rats nerve defect scale was detected, the cerebral infarction volume was evaluated by TTC method, and Western blot method was used to detect angiogenin-1 (Ang-1) expression. Results Between the four groups of rats, nerve deficit score and infarct volume were significantly higher, Ang-1 protein relative expression was significantly lower in group DC than those in group NDC (P＜0.05); neural deficit score and infarct volume were significantly lower, Ang-1 protein relative expression was significantly higher in group NDS than those in group NDC (P＜0.05); nerve deficit score and infarct volume were significantly lower, Ang-1 protein relative expression was significantly higher in group DS than those in group DC (P＜0.05). Conclusion In the rats after cerebral ischemia reperfusion, diabetes could aggravate the nerve defect, increase the volume of cerebral infarction, reduce the expression of Ang-1; sevoflurane could reduce nerve defects, reduce infarct volume, increase the expression of Ang-1. The expression of Ang-1 and degree of injury after cerebral ischemia reperfusion in rats have relationship. |
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