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| 姜黄素对内毒素休克兔急性肾损伤的影响 |
| Effects of curcumin on acute kidney injury in endotoxic shock rabbits |
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| DOI：10.12089/jca.2018.02.016 |
| 中文关键词: 姜黄素 转录因子NF-E2相关因子2 内毒素休克 急性肾损伤 |
| 英文关键词: Curcumin NF-E2-related factor 2 Endotoxic shock Acute kidney injury |
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| 中文摘要: |
| 目的 探讨姜黄素对内毒素休克兔急性肾损伤(acute kidney injury, AKI)的影响及其可能机制。方法 健康雄性新西兰大白兔40只, 2月龄, 随机分为四组: 对照组(C组)、姜黄素对照组(Cur组, 姜黄素50 mg/kg)、内毒素休克组(L组, 脂多糖5 mg/kg)和姜黄素预处理组(CurL组, 姜黄素50 mg/kg, 30 min后予脂多糖5 mg/kg), 每组10只。给予脂多糖后6 h测定血尿素氮(BUN)和肌酐(Cr)浓度并处死兔。观察肾组织病理变化并进行肾损伤评分。采用黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性, 硫代巴比妥酸法测定丙二醛(MDA)浓度, RT-PCR法检测肾组织Nrf2和HO-1 mRNA表达量, Western blot法检测肾组织中Nrf2总蛋白、Nrf2核蛋白及HO-1蛋白含量。结果 C组和Cur组肾单位结构未见明显异常。L组肾小球皱缩, 肾小管上皮细胞肿胀、空泡形成或脱落, 肾间质水肿, 大量炎性细胞浸润。CurL组病理改变较L组明显减轻。与C组比较, L组及CurL组肾损伤评分、BUN、Cr及MDA浓度明显升高, SOD活性明显降低, 肾组织Nrf2和 HO-1 mRNA表达量、Nrf2总蛋白、Nrf2核蛋白及HO-1蛋白含量明显升高(P＜0.05)。与L组比较, CurL组肾损伤评分、BUN、Cr及MDA浓度明显降低, SOD活性明显升高, 肾组织Nrf2和 HO-1 mRNA表达量、Nrf2总蛋白、Nrf2核蛋白及HO-1蛋白含量明显升高(P＜0.05)。结论 姜黄素可以减轻内毒素休克诱发兔的AKI, 其机制可能与激活Nrf2/HO-1信号通路有关。 |
| 英文摘要: |
| Objective To investigate the effects of curcumin on acute kidney injury in endotoxic shock rabbits and discuss the possible mechanism. Methods Forty healthy male New Zealand white rabbits aged 2 months were randomly divided into 4 groups: control group (group C), curcumin control group (group Cur, curcumin 50 mg/kg), endotoxic shock group (group L, LPS 5 mg/kg) and curcumin pretreatment group (group CurL, curcumin 50 mg/kg, LPS 5 mg/kg 30 minutes later), 10 in each group. The concentrations of blood urea nitrogen (BUN) and creatinine (Cr) were detected, and the rabbits were sacrificed 6 h after LPS treatment. The pathological changes of renal tissue were examined and the pathological scores were recorded. SOD activities were measured by xanthine oxidase method. MDA contents were assayed according to thiobarbituric acid method. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to investigate the mRNA levels of Nrf2 and HO-1. The expressions of Nrf2 total protein, Nrf2 nuclear protein and HO-1 protein in the renal tissue were determined by Western blot. Results No abnormal structures were visible in groups C and Cur. The histopathological changes of the kidneys included glomerular shrinkage, cellular swelling, vacuolization and desquamation in tubules, interstitial edema and massive inflammatory cells infiltration in group L. Compared with group C, the pathological manifestations was relieved obviously in group CurL. Compared with group C, the pathological score, the concentrations of BUN, Cr, and MDA were obviously increased, while SOD activity was significantly decreased, the mRNA expression of Nrf2 and HO-1, the protein levels of Nrf2 total, Nrf2 nuclear and HO-1 in the renal tissues were distinctly increased in groups L and CurL (P＜0.05). Compared with group L, the pathological score, the concentrations of BUN, Cr and MDA were obviously decreased, while SOD activity was significantly increased, the mRNA expression of Nrf2 and HO-1, the protein levels of Nrf2 total, Nrf2 nuclear and HO-1 in the renal tissues were significantly increased in group CurL (P＜0.05). Conclusion Curcumin can ameliorate acute kidney injury induced by endotoxic shock in rabbits, and its mechanism may be relate to activation of Nrf2/HO-1 signaling pathway. |
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