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| 帕瑞昔布钠对脓毒症小鼠肠黏膜屏障的保护作用 |
| Protective effect of parecoxib on intestinal barrier function of septic mice |
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| DOI： |
| 中文关键词: 帕瑞昔布钠 脓毒症 肠黏膜屏障功能 紧密连接蛋白 |
| 英文关键词: Parecoxib Sepsis Intestinal barrier function Tight junction protein |
| 基金项目: |
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| 中文摘要: |
目的：研究帕瑞昔布钠对脓毒症小鼠肠黏膜屏障功能的影响及可能机制。
方法：21只C57BL/6小鼠随机分为三组: 假手术组(Sham组)、脓毒症模型组(CLP组)、脓毒症模型+帕瑞昔布钠2 mg/kg治疗组(P组), 每组7只。术后24 h用襻环结扎法检测各组小鼠小肠通透性。另21只C57BL/6小鼠随机分为三组, 分组及治疗同前, 术后24 h处死小鼠, 收集小鼠回肠。小肠组织行HE染色观察肠病理损伤。采用Western bolt法检测小肠ZO-1、Occludin、Claudin-1等紧密连接蛋白的表达。采用ELISA法检测小肠黏膜IL-6、PGE2的浓度。
结果：与Sham组比较, CLP组小鼠小肠明显损伤,门静脉血内FD4浓度明显升高,小肠黏膜内ZO-1、Occludin、Claudin-1蛋白表达明显减少、IL-6与PGE2浓度明显升高(P<0.05)。与CLP组比较, P组小鼠小肠损伤明显减轻（P<0.05）,门静脉血内FD4浓度明显降低（P<0.05）,小肠黏膜内各蛋白表达水平明显增加、IL-6与PGE2浓度明显降低(P<0.05)。
结论：帕瑞昔布钠治疗可以明显降低脓毒症导致的肠黏膜屏障功能损伤, 其机制可能与降低肠组织炎症水平, 增加肠紧密连接蛋白表达相关。 |
| 英文摘要: |
Objective: To observe the effect of parecoxib on intestinal barrier function of septic mice.
Methods: Sepsis was induced by cecal ligation and puncture (CLP) model. Twenty-one male C57BL/6 mice were randomly divided into three groups (n=7 in each group): group Sham, group CLP, group P (parecoxib 2 mg/kg was administered via gastric tube 2 h after CLP). In vivo intestinal permeability was measured using an in vivo ligated loop model 24 h after surgery. Twenty-one male C57BL/6 mice were randomly divided into three groups as before. The small intestine tissue sample was harvested 24 h after surgery. The intestinal pathological changes were observed under light microscope. The expression of tight junction proteins ZO-1, Occludin, and Claudin-1 in the ileum were measured by Western blot. IL-6 and PGE2 level in the ileum were measured by ELISA.
Results: Compared with group Sham, the intestinal permeability was significantly increased and there was a significant intestinal pathological injury in group CLP. IL-6 and PGE2 level in the ileum was significantly increased and the expression of tight junction protein ZO-1, Occludin, and Claudin-1 in the ileum were reduced in the group CLP (P<0.05). Compared with the group CLP, intestinal permeability and pathological injury was significantly reduced in the group P. The levels of IL-6 and PGE2 were significantly decreased (P<0.05), the expression of ZO-1, Occludin, and Claudin-1 were up-regulated in group P (P<0.05).
Conclusion: Parecoxib can decrease the levels of proinflammatory factors and up-regulate the expression of tight junction to reverse intestinal barrier dysfunction caused by sepsis in mice. |
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