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| 七氟醚对新生大鼠下丘脑-垂体-肾上腺皮质轴发育的影响 |
| Effects of sevoflurane exposure on the hypothalamic-pituitary-adrenal axis develoopment in neonatal rat |
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| DOI： |
| 中文关键词: 七氟醚 下丘脑-垂体-肾上腺皮质轴 布美他尼 应激 |
| 英文关键词: Sevoflurane Hypothalamic-pituitary-adrenal axis Bumetanide Stress |
| 基金项目:国家自然科学基金 (81301144)；国家留学基金(201608430251) |
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| 中文摘要: |
目的：研究新生期七氟醚暴露对下丘脑-垂体-肾上腺皮质 (hypothalamic-pituitary-adrenal, HPA) 轴发育及功能的影响。
方法：新生雄性SD大鼠54只, 6日龄, 随机分为三组: 对照组(C组) 、七氟醚组(S组)和布美他尼+七氟醚组(BS组), 每组18只。C组: 吸入100％氧气6 h;S组: 吸入2.1％七氟醚6 h;BS组: 吸入2.1％七氟醚6 h, 在麻醉前30 min和麻醉后3 h注射布美他尼。6 h后, 每组随机选取6只处死收集血清,采用ELISA法测定皮质酮浓度;各组剩余大鼠于第60天行高架十字迷宫测试,第65天行束缚应激30 min, 并在应激前后取尾静脉血,采用ELISA法测定血清皮质酮浓度。
结果：麻醉后S组血清皮质酮浓度明显高于C组(P＜0.01);BS组血清皮质酮浓度明显低于S组(P＜0.01)。S组进入开放臂次数明显少于C组(P＜0.05), 开放臂停留时间明显短于C组(P＜0.05), 应激前后血清皮质酮浓度明显高于C组(P＜0.05或P＜0.01);BS组进入开放臂次数明显多于S组, 开放臂停留时间明显长于S组(P＜0.05)，应激前后血清皮质酮浓度明显低于S组(P＜0.05)。
结论：新生期七氟醚麻醉可导致HPA轴功能亢进, 其机制与GABAA受体相关。 |
| 英文摘要: |
Objective: To investigate the effects of sevoflurane exposure on the hypothalamic-pituitary-adrenal (HPA) axis development in neonatal rats.
Methods: Fifty-four SD rats (P6) were randomly divided into three groups: control group (group C), sevoflurane group (group S) and bumetanide+sevoflurane group (group BS), n=18 in each group. In group C, rats were treated with pure oxygen for 6 hours and rats in group S received 2.1％ evoflurane anesthesia for 6 h. Rats in group BS also received 2.1％ sevoflurane anesthesia for 6 h, but they were injected with bumetanide before 30 min and at 3 h during the anesthesia. 6 rats from each group were randomly selected and the tail blood was collected to measure the orticosterone in the serum using ELISA. Remaining rats in each group were normally housed to 60. A elevated plus maze (EPM) were employed to investigate the anxiety-like behavior, and estraint stress were used to evaluate the function of HPA axis.
Results: In P6 rats, evoflurane significantly increased the serum corticosterone level (P＜0.01) while bumetanide retreatment decreased corticosterone level (P＜0.01). In P60 rats, neonatal exposure to evoflurane decreased the visits and time spent in the open arms of EPM (P＜0.05), increased the corticosterone level pre-stress and post-stress (P＜0.05 or P＜0.01). Pretreatment of bumetanide normalized serum corticosterone level and the anxiety behavior in the EPM.
Conclusion: Neonatal sevoflurane exposure caused hyperactive HPA axis in rats, hich is normalized by bumetanide pretreatment through blocking GABAA receptors. |
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