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| 2型糖尿病小鼠周围神经病变模型的建立及皮下神经纤维密度的检测 |
| Establishing mouse model of type 2 diabetes peripheral neuropathy and measuring its intra-epidermal nerve fiber density |
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| DOI： |
| 中文关键词: 2型糖尿病 糖尿病周围神经病变 小鼠模型 皮下神经纤维密度检测 |
| 英文关键词: Type 2 diabetes Diabetic peripheral neuropathy Mouse model Intra-epidermal nerve fiber density |
| 基金项目:上海市科委医学引导项目基金资助(134119b2700) |
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| 中文摘要: |
目的：建立2型糖尿病小鼠周围神经病变模型, 并对该模型的皮下神经纤维密度(intra-epidermal nerve fiber density,IENFD)进行检测。
方法：C57BL6雄性小鼠24只, 随机分为四组, 每组6只: HS组采用高脂饲料喂养24周+链脲佐菌素STZ(120 mg/kg)单次注射;H组采用高脂饲料喂养24周+缓冲液注射;S组普通饲料喂养24周+STZ(120 mg/kg)单次注射;C组普通饲料喂养24周+缓冲液注射。四组小鼠均以24周为实验终点, 测定随机血糖、胰岛素抵抗指数(HOMA-IR)、机械痛阈和IENFD。
结果：第24周时HS组随机血糖、HOMA-IR明显高于H、S、C组(P＜0.01), 机械痛阈明显低于H、S、C组(P＜0.05)，IENFD明显低于S组和C组(P＜0.05);H组随机血糖、HOMA-IR明显高于C组(P＜0.01), 机械痛阈与S组、C组差异无统计学意义, IENFD与HS组差异无统计学意义。
结论：高脂饲料喂养联合中等剂量STZ成功建立了2型糖尿病小鼠周围神经病变模型,小鼠皮下神经纤维密度明显下降。 |
| 英文摘要: |
Objective: To establish mouse model of type 2 diabetes peripheral neuropathy and measure its intra-epidermal nerve fiber density (IENFD).
Methods: Male C57BL6 mouse were randomly divided into four groups: group HS (n=6): high-fat diet+single treptozotocin intraperitoneal injection (120 mg/kg); group H (n=6): high fat diet+buffer injection; group S (n=6): standard chow diet+single streptozotocin intraperitoneal injection (120 mg/kg); group C (n=6): standard chow diet+buffer injection. The 24th week was the end point of the experiment, and random glucose, homeostasis model assessment of insulin resistance (HOMA-IR), mechanical threshold, and IENFD were measured.
Results: Group HS had significantly higher random glucose and HOMA-IR than other groups (P＜0.01), had significantly lower mechanical threshold than other groups (P＜0.05), had significantly lower IENFD than groups S and C at 24th week (P<0.05); group H had significantly higher random glucose and HOMA-IR than group C at 24th week (P＜0.01), had no significant difference in mechanical threshold compared with group S and group C, and had no significant difference in IENFD compared with group HS.
Conclusion: A mouse model of type 2 diabetes peripheral neuropathy was successfully established, and the IENFD was found to be decreased significantly. |
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