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| 舒芬太尼预处理联合咪达唑仑后处理对大鼠心肌缺血-再灌注损伤的影响 |
| Influences of sufentanil and midazolam coprocessing on myocardial injury in rats during myocardial ischemia-reperfusion |
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| DOI： |
| 中文关键词: 舒芬太尼 咪达唑仑 缺血-再灌注模型 心肌损伤 心肌梗死 |
| 英文关键词: Sufentanil Midazolam Ischemia-reperfusion model Myocardial injury Myocardial infarction |
| 基金项目:2016军队后勤科研项目(CCD16J001) |
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| 中文摘要: |
目的：探讨舒芬太尼预处理联合不同剂量咪达唑仑后处理对大鼠心肌缺血-再灌注(ischemia-reperfusion, IR)损伤的影响。
方法：SPF级健康雄性SD大鼠42只, 随机分为七组, 每组6只。A组: 行假手术, 穿线不结扎;B0～B5组: 构建大鼠心肌IR模型, 均结扎0.5 h, 再灌注2 h。其中B0组: 未经舒芬太尼和/或咪达唑仑处理;B1组: 经舒芬太尼3 μg/kg预处理;B2组: 经咪达唑仑0.1 mg/kg后处理;B3组: 经咪达唑仑0.3 mg/kg后处理;B4组: 经舒芬太尼1.5 μg/kg预处理联合咪达唑仑0.05 mg/kg后处理;B5组: 经舒芬太尼1.5 μg/kg预处理联合咪达唑仑0.15 mg/kg后处理。经颈静脉, 舒芬太尼于缺血前注入, 咪达唑仑于缺血后注入。心肌IR结束后测定血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性和血清丙二醛(MDA)浓度, 及炎性因子TNF-α和IL-6浓度。再灌注2 h取各组大鼠心脏, 测算心肌梗死严重程度(IS/AAR), 采用Western blot法检测心肌组织中Bcl-2、Bax及cleaved-caspase-3蛋白含量。
结果：B0～B5组CK-MB和LDH活性、MDA、TNF-α和IL-6浓度、Bax及cleaved-caspase 3蛋白含量均明显高于A组(P＜0.05),B1～B5组均明显低于B0组(P＜0.05), B3和B4组明显低于B2组(P＜0.05),B5组明显低于B1～B4组(P＜0.05);B1～B5组IS值及IS/AAR(％)值均明显低于B0组(P＜0.05),B3和B4组明显低于B2组(P＜0.05),B5组明显低于B1～B4组(P＜0.05);B0～B5组SOD活性及Bcl-2含量均明显低于A组(P＜0.05),B1～B5组均明显高于B0组(P＜0.05),B3和B4组明显高于B2组(P＜0.05),B5组明显高于B1～B4组(P＜0.05)。与B0组比较,B5组CK MB、LDH活性明显下降,SOD活性明显上升,MDA、TNF-α、IL-6浓度明显下降,IS/AAR明显下降,Bcl-2含量增至B0组的2.25倍,Bax含量降至B0组的54.89％,cleaved-caspase-3含量降至B0组的49.67％。
结论：舒芬太尼预处理联合咪达唑仑后处理可明显减轻大鼠心肌IR损伤, 对心肌的保护作用明显优于单独使用时。 |
| 英文摘要: |
Objective: To explore the influences of sufentanil and different dose midazolam coprocessing on myocardial injury in rats during myocardial ischemia-reperfusion (IR).
Methods: Forty two healthy male SD rats were selected and randomly divided into 7 groups including, group A: rats were treated with sham operation; groups B0-B5: rat IR model was constructed; group B0: rats were untreated with sufentanil or midazolam; group B1: rats were per-treated with 3 μg/kg sufentanil; group B2: rats were post-treated with 0.1 mg/kg midazolam; group B3: rats were post-treated with 0.3 mg/kg midazolam; group B4: rats were per-treated with 1.5 μg/kg sufentanil and post-treated with 0.05 mg/kg midazolam; group B5: rats were per-treated with 1.5 μg/kg sufentanil and post-treated with 0.15 mg/kg midazolam. Sufentanil was injected through cervical vein before ischemia. Midazolam was injected through cervical vein after ischemia. After reperfusion, arterial blood was collected for detecting the activity of CK-MB, LDH or SOD, and the concentration of MDA, TNF-α, or IL-6. Rat hearts were picked off to measure the severity of myocardial infarction (IS/AAR). The expressions of Bax, cleaved-caspase-3 and Bcl-2 in myocardial tissues were detected by Western blots.
Results: CK-MB activity, LDH activity, MDA concentration, TNF-α level, IL-6 level, Bax and cleaved-caspase 3 expression levels in groups B0-B5 were obviously higher than those in group A (P＜0.05), in groups B1-B5 were obviously lower than those in group B0 (P＜0.05), in groups B3 and B4 were obviously lower than those in group B2 (P＜0.05), in group B5 were bviously lower than those in groups B1-B4 (P＜0.05); values of IS and IS/AAR (％) in groups B1-B5 were obviously lower than those in group B0 (P＜0.05), in groups B3 and B4 were obviously ower than those in group B2 (P＜0.05), in group B5 were obviously lower than those in groups B1-B4 (P＜0.05); SOD activity and Bcl-2 expression level in groups B0-B5 were obviously lower than those in group A (P＜0.05), in groups B1-B5 were obviously higher than those in group B0 (P＜0.05), in groups B3 and B4 were obviously higher than those in group B2 (P＜0.05), in group B5 were obviously higher than those in groups B1-B4 (P＜0.05). CK-MB declined when compared with group B0; LDH declined; SOD rose; MDA declined; TNF-α declined; IL-6 declined; IS/AAR declined; Bcl-2 expression rose to 2.25 fold; Bax expression dropped to 54.89％; leaved-caspase-3 expression dropped to 49.67％.
Conclusion: Sufentanil retreatment combined with midazolam aftertreatment can significantly alleviate rat myocardial IR injury, which plays more effective cardioprotective effects than being used alone. |
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