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| 脑室内注射orexin-A和orexin-B对下丘脑组胺释放的影响 |
| Effect of microinjection of orexin-A and orexin-B into cerebral ventricle on the release of histamine in hypothalamus |
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| DOI： |
| 中文关键词: orexin-A orexin-B 组胺 |
| 英文关键词: Orexin-A Orexin-B Histamineorexin |
| 基金项目:国家自然科学基金（81371510）；国家自然科学青年基金（81401138） |
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| 中文摘要: |
目的 观察脑室内微注射orexin-A和orexin-B对下丘脑组胺释放的影响，为orexins促麻醉觉醒的机制提供新的解释。
方法 成年雄性SD大鼠随机分为三组：生理盐水组、orexin-A组和orexin-B组。在立体定位仪下将微透析管置入下丘脑结节乳头体核区。实验分为两部分：先收集脑室内分别注射orexin-A、orexin-B 1 nmol和5 nmol后1、2 h和3 h的微透析液(n=5)，采用高效液相色谱法(HPLC)检测微透析液中组胺含量的时程变化。1周后，各组大鼠分别注入不同剂量的orexin-A和orexin-B(10、15 nmol和20 nmol，n=5)，收集微注射后1 h的透析液，检测组胺释放的剂量效应。在立体定位仪下结节乳头体核置入微注射管，1周后给予1.4％异氟醚(1 MAC)麻醉30 min，各组大鼠分别微注射生理盐水、orexin-A和orexin-B 0.3μl(n=6)，记录翻正反射恢复时间。
结果 与生理盐水组比较，脑室内微注射orexin-A 1nmol2 h后下丘脑组胺含量明显增加，但同样剂量的orexin-B无明显作用，注射orexin-A和orexin-B 5nmol后2、3 h组胺含量均明显增加(P<0.01)。脑室内微注射10nmol、15 nmol和20 nmol的orexin-A和orexin-B后1h组胺含量明显高于生理盐水，且20nmol含量最高(P<0.05)。结节乳头体核微注射orexin-A后翻正反射恢复时间明显短于生理盐水(P<0.01)，微注射orexin-B后无明显作用。
结论 脑室内微注射orexin-A和orexin-B均可促进下丘脑组胺递质的释放，但orexin-A的作用更强。结节乳头体核微注射orexin-A促进异氟醚麻醉的觉醒。 |
| 英文摘要: |
Objective To provide new evidences for understanding the mechanisms of promotive role of orexins in anesthetic emergence and the effect of microinjection of orexin-A/orexin-B into cerebral ventricle on the release of histamine.
Methods Male SD rats were randomly divided into saline (control), orexin-A and orexin-B groups. The microdialysis probe was inserted into hypothalamus under stereotaxic apparatus. The perfused fluid from the area of hypothalamic tuberomammillary nucleus was collected using in vivo microdialysis at 1 h, 2 h and 3 h after 1 nmol or 5 nmol orexin-A or orexin-B microinjection into the cerebral ventricle (n=5each).The concentrations of histamine at eachtime point in dialysates of perfused fluid were detected by high performance liquid chromatography (HPLC) to analyze its dynamic changes. Afterone week, each group was microinjected with 10 nmol, 15 nmol and 20 nmol orexin-A and orexin-B (n=5) into the cerebral ventricle respectively, dialysates was collect and histamine was detected at 1 h to analyze its dosage response. After one week, each group was microinjected 0.3 μl saline orexin-A and orexin-B (n=6) into the tuberomammillary nucleus.
Results Compared with the control group, microinjection of 1 nmol orexin-A significantly increased histamine release at 1 h, but the same dose of orexin-B had no such effect, 5 nmol of orexin-A or orexin-B injections significantly facilitated histamine release at 2 h and 3 h (P<0.01). Microinjection of 10 nmol, 15 nmol and 20 nmol orexin-A and orexin-B into ventricle caused an significant increase of histamine release at 1 h while the effect was the strongest in 20 nmol (P<0.05). Compared with the control group, microinjection of orexin-A significantly decreased time of the righting reflex (P<0.01), but the same dose of orexin-B had no such effect.
Conclusion Microinjection of both orexin-A or orexin-B into cerebral ventricle could promote the release of histamine, while the effect of orexin-A was stronger. Microinjection orexin-A into tuberomammillary nucleus significant facilitated recovery from isoflurane. |
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