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| 神经病理性疼痛致抑郁大鼠海马促炎细胞因子含量的变化 |
| Changes of hippocampal levels of pro-inflammatory cytokines in rats with depression induced by neuropathic pain |
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| DOI： |
| 中文关键词: 神经病理性疼痛 抑郁 炎症因子 海马 |
| 英文关键词: Neuropathic pain Depression Inflammatory cytokines Hippocampus |
| 基金项目:国家自然科学基金（81571083） |
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| 摘要点击次数: 3140 |
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| 中文摘要: |
目的 观察神经病理性疼痛致抑郁大鼠海马促炎细胞因子含量的变化,并探讨其与抑郁严重程度的关系。
方法 成年雄性SD大鼠28只,随机分为两组:假手术组(Sham组)和保留性坐骨神经损伤组(SNI组),每组14只。于术前1 d及术后7、14、21 d测试机械痛阈值(MWT);于术前1～3 d测试糖水偏好、强迫游泳实验基础值;术后21～23 d进行糖水偏好实验、强迫游泳实验;行为学测试结束后取海马组织,采用ELISA法检测海马中IL-1β、IL-6、TNF-α的含量,并对其与糖水偏好的相关性进行分析。
结果 与Sham组比较,SNI组大鼠在术后7、14、21 d MWT明显降低(P<0.001),术后21 d糖水偏好明显降低(P<0.01),术后23 d强迫游泳不动时间明显延长(P<0.05);术后21 d海马组织IL-6、TNF-α含量明显升高(P<0.05或P<0.01),而IL-1β含量差异无统计学意义;SNI组海马组织IL-1β（r2=0.60,P<0.01）、IL-6（r2=0.55,P<0.01）、TNF-α（r2=0.60,P<0.01）含量与糖水偏好呈明显负相关。
结论 神经病理性疼痛致抑郁大鼠海马促炎细胞因子IL-6、TNF-α含量升高,且与抑郁严重程度呈负相关。 |
| 英文摘要: |
Objective To observe changes of hippocampal levels of pro-inflammatory cytokines in rats with neuropathic pain-induced depression, and to explore the relationship between hippocampal levels of pro-inflammatory cytokines and severity of depression.
Methods Twenty-eight adult male rats were randomly divided into two groups: group sham (n=14) and spared nerve injury (group SNI) (n=14). Mechanical withdrawal thresholds (MWT) were measured 1 day before and 7, 14 and 21 days after operation. Sucrose preference test and forced swim test were tested 1-3 days before and 21-23 days after operation. After test, hippocampus was collected. The hippocampal levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) were measured by ELISA. Correlational relationships between hippocampal levels of pro-inflammatory cytokines and sucrose preference were evaluated.
Results Compared with group sham, MWT was decreased 7, 14, 21 days after operation (P<0.001). Group SNI displayed decreased sucrose preference 21 days after operation (P<0.01) and increased immobility time in FST 23 days after operation (P<0.05). The levels of IL-6 and TNF-α were increased in hippocampus (P<0.05 or P<0.01). No significant difference was observed in IL-1β. The hippocampal levels of IL-1β (r2=0.60,P<0.01), IL-6 (r2=0.55,P<0.01) and TNF-α(r2=0.60,P<0.01) were negatively correlated with sucrose preference.
Conclusion The hippocampal levels of pro-inflammatory cytokines IL-6 and TNF-α are increased in rats with depression induced by neuropathic pain, and the levels of hippocampal pro-inflammatory cytokines are negatively correlated with the severity of depression. |
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