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| 七氟醚后处理对大鼠脑缺血-再灌注时氧化应激和炎症反应的影响 |
| Effects of sevoflurane post conditioning on oxidative stress and inflammatory reaction during rat cerebral ischemia reperfusion |
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| DOI： |
| 中文关键词: 脑缺血-再灌注 吸入麻醉 后处理 氧化应激 炎症 |
| 英文关键词: Cerebral ischemia-reperfusion Iinhalation anesthesia Post-conditioning Oxi dative stress Inflammation |
| 基金项目:江西省卫计委项目 (20165284) |
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| 中文摘要: |
| 目的 评价七氟醚后处理对大鼠脑缺血-再灌注时氧化应激及炎症反应的影响，以探讨其脑保护机制。方法 健康雄性清洁级SD大鼠36只，12～14周龄，体重220～260 g，采用随机数字表法分为假手术组 (Sham组)、脑缺血-再灌注组 (IR组)和脑缺血-再灌注+七氟醚后处理组 (SPC组)，每组12只。制备大鼠脑缺血-再灌注损伤模型，缺血30 min后再灌注24 h。Sham组不阻塞大脑中动脉;IR组:建立脑缺血-再灌注损伤模型;SPC组于再灌注即刻给予2.6％七氟醚吸入15 min。再灌注末处死各组大鼠，断头取出脑组织。采用Western blot法检测Iba-1和HO-1蛋白含量；并测定脑组织中活性氧(ROS)含量，丙二醛 (MDA)、TNF-α、IL-1β浓度和超氧化物歧化酶(SOD)活性。结果 IR组和SPC组脑皮质Iba-1蛋白含量明显高于Sham组(P<0.05)，SPC组Iba-1蛋白含量明显低于IR组(P<0.05)。与Sham组比较，IR组和SPC组ROS含量和MDA、TNF-α、IL-1β浓度明显升高，SOD活性和HO-1蛋白含量明显降低(P<0.05)。SPC组ROS含量和MDA、TNF-α、IL-1β浓度明显低于IR组，SPC组SOD活性和HO-1蛋白含量明显高于IR组(P<0.05)。结论 七氟醚后处理能抑制脑缺血-再灌注时诱发的小胶质细胞激活，减轻脑组织氧化应激及炎症反应，从而减轻脑缺血-再灌注损伤，发挥其脑保护作用。 |
| 英文摘要: |
| Objective To investigate the effects of sevoflurane post-conditioning on oxidative stress and inflammatory reaction during rat cerebral ischemia-reperfusion, and to explore its cerebral protective mechanism. Methods Thirty-six health male Sprague-Dawley rats (aged 12-14 weeks, weighing 220-260 g) were randomly divided into 3 groups (n=12 each): sham control group (group Sham), cerebral ischemia-reperfusion group (group IR), sevoflurane post-conditioning group (group SPC). Cerebral ischemia-reperfusion model was established, ischemia for 30 min followed by reperfusion 24 h. Rat middle cerebral artery was not occluded in group Sham. Cerebral ischemia-reperfusion model was established in group IR. Group SPC was subjected to 2.6％ sevoflurane for 15 min in the beginning of reperfusion. At the end of reperfusion, rats were cut off the head to take out the brain tissue. The expression level of Iba-1 and HO-1 proteins was measured by western blot. The levels of reactive oxygen species (ROS), malondialdehyde (MDA), TNF-α, IL-1β and the activity of superoxide dismutase (SOD) were evaluated. Results Compared with group Sham, the expression of cerebral cortex Iba-1 protein was higher than that in groups IR and SPC (P<0.05), the expression of Iba-1 protein in group SPC was lower than that in group IR (P<0.05). Compared with group Sham, the contents of ROS, MDA, TNF-α and IL-1β were increased in groups IR and SPC (P<0.05), but the activity of SOD and expression of HO-1 protein were decreased (P<0.05). And the contents of ROS, MDA, TNF-α and IL-1β in group SPC were less than those in group IR, the activity of SOD and expression of HO-1 protein in group SPC were higher than those in group IR. Conclusion Sevoflurane post-conditioning can mitigate the microglia activation, reduce cerebral oxidative stress and inflammation, thus protect rat cerebral against ischemia reperfusion injury. |
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