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| 电针足三里穴对依托咪酯麻醉大鼠急性应激反应的影响 |
| Effect of electroacupuncture at tsusanli on regulation of stress response under etomidate anesthesia in rats |
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| DOI： |
| 中文关键词: 电针 依托咪酯 应激 足三里穴 c-fos蛋白 |
| 英文关键词: Electroacupuncture Etomidate Stress Tsusanli c-fos Protein |
| 基金项目:2012年广西高校学校科研项目(201204LX243);2014年桂林市科学研究与技术开发计划课题(20140310-2-3) |
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| 中文摘要: |
| 目的 探讨电针足三里穴对依托咪酯麻醉大鼠应激反应的影响。方法 选择雄性SD大鼠60只，体重280～310 g，随机分为六组：空白对照组(C组)、模型组(M组)、依托咪酯60 mg/kg组(E1组)、依托咪酯30 mg/kg组(E2组)、依托咪酯60 mg/kg联合电针组(EA1组)和依托咪酯30 mg/kg复合电针组(EA2组)，每组10只。C组大鼠不进行任何麻醉手术操作，其余五组均建立低位尾干切断模型。建模后，E1组和EA1组大鼠腹腔注射依托咪酯60 mg/kg，E2组和EA2组大鼠腹腔注射依托咪酯30 mg/kg。EA1组和EA2组大鼠于麻醉状态行双侧足三里穴治疗30 min (频率2/100 Hz，疏密波，电流强度1 mA)。采用ELISA法检测血清ACTH和Cor的浓度，Western blot法检测下丘脑室旁核组织c-fos蛋白的含量。结果 M组ACTH、Cor浓度和c-fos蛋白含量明显高于C组(P<0.05)；E1组、E2组、EA1组、EA2组ACTH、Cor浓度和c-fos蛋白含量明显低于M组(P<0.05)；E2组、EA1组ACTH、Cor浓度和c-fos蛋白含量明显高于E1组(P<0.05)；EA2组ACTH浓度和c-fos蛋白含量明显低于E2组(P<0.05)。 结论 电针大鼠足三里穴可双向调节依托咪酯对急性应激反应的影响，其机制可能与其调节下丘脑c-fos蛋白水平有关。 |
| 英文摘要: |
| Objective To explore the effect of electroacupuncture (EA) at tusanli (ST36) on regulation of stress response under different doses of etomidate anesthesia in rats. Methods Sixty male Sprague-Dawley rats, weighing 280-310 g, were randomly divided into control group (group C), model group (group M), etomidate 60 mg/kg group (group E1), etomidate 30 mg/kg group (group E2), etomidate 60 mg/kg combined with EA group (group EA1) and etomidate 30 mg/kg combined with EA group (group EA2), n=10 in each group. All groups received inferior caudal trunk transection at the level between sacral spinal nerve 3 and 4 (S3, S4) to prepare acute stress response model except group C. Group M received no others treatment. The rats in group E1, group EA1, group E2 and group EA2 were intraperitoneally injected with 60, 60, 30 and 30 mg/kg etomidate, respectively. Group EA1 and group EA2 received EA ST36. The points were stimulated at a frequency of 2/100 Hz with 1 mA output and a dilatational wave, which lasted for 30 min. ACTH and Cor levels were measured by ELISA. The c fos protein expression in hypothalamic tissue was examined by Western blot. Results Compared with group C, ACTH and Cor levels, and hypothalamic expression of c-fos protein in group M were significantly increased (P<0.05). Compared with group M, serum ACTH and Cor levels, and hypothalamic expression of c-fos protein in groups E1, E2, EA1 and EA2 were significantly decreased (P<0.05). Compared with group E1, serum ACTH and Cor levels, and hypothalamic expression of c-fos protein were significantly higher in groups E2 and EA1 (P<0.05). Compared with group E2, serum ACTH level and hypothalamic expression of c-fos protein were significantly lower in group EA2 (P<0.05). Conclusion EA at ST36 regulating stress response under etomidate anesthesia in rats is effective and two-way, and the mechanism may be due to the release of neurotransmitters induced c-fos protein in hypothalamus. |
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