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| 虎杖苷对脓毒症急性肾损伤大鼠炎症反应和氧化应激的影响 |
| Effect of polydatin on inflammatory response and oxidative stress in rats with sepsis-induced acute kidney injury |
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| DOI： |
| 中文关键词: 虎杖苷 炎症反应 氧化应激 脓毒症 急性肾损伤 |
| 英文关键词: Polydatin Inflammatory response Oxidative stress Sepsis Acute kidney injury |
| 基金项目:福建省自然科学基金(2016J01451);福建省科技创新联合资金项目(2016Y9012) |
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| 中文摘要: |
| 目的 观察虎杖苷在脓毒症急性肾损伤(acute kidney injury，AKI)大鼠中的抗炎和抗氧化应激作用。方法 SD大鼠72只，体重180～220 g，随机分为四组: 假手术对照组(Sham组)；盲肠结扎穿刺术(CLP)+生理盐水组(CN组)；CLP+溶媒组(CV组)；CLP+虎杖苷组(CD组)，每组18只。对CN、CV和CD组大鼠施行CLP，模拟脓毒症AKI动物模型，Sham组大鼠盲肠既不被结扎也不被穿孔，余步骤则与CLP组相同，未行其他任何处理。CLP术后6、12、18 h，CN、CV和CD组经大鼠尾静脉分别注射生理盐水、溶媒、虎杖苷30 mg/kg。在CLP术后24 h记录血清肌酐(Cr)和尿素氮(BUN)等肾功能指标，观察肾脏组织病理形态改变并进行肾小管损伤评分，检测血清TNF-α、IL-1β和IL-6浓度，测定肾组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)、谷胱甘肽(GSH)浓度。结果 与Sham组比较，CN组和CV组血清Cr、BUN、TNF-α、IL-1β和IL-6浓度、肾小管损伤评分和肾组织MDA浓度明显升高(P<0.01)，肾组织SOD活性、GSH浓度明显降低(P<0.01)。与CN组和CV组比较，CD组血清Cr、BUN、IL-1β和IL-6浓度、肾小管损伤评分和肾组织MDA浓度明显降低(P<0.05)，肾组织SOD活性、GSH浓度明显升高(P<0.05)。结论 盲肠结扎穿刺术导致的脓毒症可引起急性肾损伤，虎杖苷可通过显著的抗炎和抗氧化应激作用，减轻肾组织损伤，改善肾功能。 |
| 英文摘要: |
| Objective To determine if polydatin inhibited oxidative stress and inflammatory response in rats with sepsis-induced acute kidney injury (AKI). Methods Seventy-two rats (weighing 180-220 g) were randomly divided into the following groups: sham group, cecal ligation and puncture (CLP) CLP+normal saline group (group CN), group CLP+vehicle (group CV), and group CLP+polydatin (group CD) (n=18 each). Rats in groups CN, CV and CD underwent CLP to mimic sepsis-induced AKI. In sham group, the cecum was not ligated or punched, and the remaining procedures were the same as in group CLP. Normal saline, vehicle, and 30 mg/kg polydatin were administered at 6, 12, and 18 hours after CLP via the tail vein. At 24 hour post CLP, two clinically used markers of AKI, blood urea nitrogen (BUN), and creatinine (Cr) were tested, pathological changes of kidney tissue was observed under light microscopy in each group. Renal tubular damage assessment was carried out. Malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) content of renal tissue, serum cytokines such as TNF-α, IL-1β and IL-6, were also measured in each group at 24 hours after CLP. Results Compared with sham group, multiple indexes such as BUN, Cr, tubular injury scores, MDA content of renal tissue, and serum cytokines incluing TNF-α, IL-1β and IL-6 increased significantly (P<0.01), while SOD and GSH levels of renal tissue significantly decreased in groups CN and CV (P<0.01). Compared with groups CN and CV, the indicators such as BUN, Cr, tubular injury scores, MDA content of renal tissue, and serum cytokines such as IL-1β and IL-6 significantly decreased (P<0.05); while SOD and GSH levels of renal tissue significantly increased (P<0.05). Conclusion Sepsis caused by sepsis cecal ligation and puncture can cause acute kidney injury. Polydatin could alleviate kidney damage by attenuating systemic inflammatory response and inhibiting oxidative stress of renal tissue. |
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