文章摘要
异氟醚对老年大鼠认知功能及大脑颞叶GABA受体1和NMDA受体2B表达的影响
Expression of GABAR1 and NMDAR2B in aged rat cerebral temporal lopes after isoflurane inhalation
  
DOI：
中文关键词: 异氟醚  Morris水迷宫  γ-氨基丁酸受体1  N-甲基-D-天冬氨酸受体2B  颞叶
英文关键词: Isoflurane  Morris water maze  GABAR1  NMDAR2B  Temporal lope
基金项目:河北北方学院自然科学研究计划项目 (120176)
作者单位
曹高亚 075000,张家口市,河北北方学院 
吴倍 075000,张家口市,河北北方学院 
邢珍 075000,张家口市,河北北方学院 
焦保良 075000,张家口市,河北北方学院 
李福龙 075000,张家口市,河北北方学院 
滕金亮 075000,张家口市,河北北方学院 
王新生 075000,张家口市,河北北方学院 
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中文摘要:
      目的 观察吸入不同浓度异氟醚后老年大鼠空间记忆以及脑右侧颞叶γ-氨基丁酸受体1 (GABAR1)和N-甲基-D-天冬氨酸受体2B(NMDAR2B)表达的变化。方法 雄性SD老年大鼠90只，9月龄，随机分为对照组 (C组，n=10)和实验组 (n=80)。C组吸入室温空气，实验组按照异氟醚浓度和吸入持续时间分为S1组 (1.5%-2 h)、S2组 (2.5%-2 h)、S3组 (1.5%-4 h)和S4组 (2.5%-4 h)。各组均分为两组分别在麻醉后1、7 d进行Morris水迷宫实验，采用RT-PCR技术和免疫荧光技术检测右侧脑颞叶GABAR1和NMDAR2B的mRNA表达和蛋白含量。结果 与对照组比较，异氟醚吸入1 d后S1、S2、S3、S4组的空间记忆能力伴随着药物浓度升高和吸入持续时间延长出现持续下降，颞叶中GABAR1的mRNA表达和蛋白含量明显升高，NMDAR2B的mRNA表达和蛋白含量明显降低(P<0.01)。异氟醚吸入7 d后，S1、S2、S3组大鼠的空间记忆能力与对照组差异无统计学意义，S4组明显低于对照组和其他三组 (P<0.01);S1、S2、S3组GABAR1和NMDAR2B的mRNA表达和蛋白含量与对照组差异无统计学意义；与对照组比较，S4组GABAR1的mRNA表达和蛋白含量明显升高，NMDAR2B的mRNA表达和蛋白含量明显降低(P<0.01)。结论 持续吸入异氟醚对空间记忆能力的影响显著，而且长时间高浓度的吸入方式造成的影响时间较长。这种影响与脑颞叶中GABAR1和NMDAR2B等神经递质受体的表达相关。
英文摘要:
      Objective To investigate the effects of different concentration and inhalation duration time of isoflurane on cognitive performance and the expression of GABAR1 and NMDAR2B in aged SD rat cerebral temporal lopes. Methods Aged male SD rats (9 months) were randomly divided into control group (n=10) and test group (n=80). The control group received air at room tempreture. Test groups were divided into four groups: group S1 (1.5%-2 h),group S2 (2.5%-2 h),group S3 (1.5%-4 h),group S4 (2.5%-4 h)according to isoflurane concentration and inhalation duration time. Every group was equally divided into two groups and Morris water maze test was performed day 1 and day 7 after isoflurane inhalation. Then the right temporal lobe was gathered and the mRNA transcription and protein expression of GABAR1 and NMDAR2B were detected by RT-PCR and Immunofluorescence technique. Results One day after isoflurane inhalation, accompanied with increased isoflurane concentration and inhalation duration, the spatial memory ability of every test group decreased continually, and the mRNA transcription and protein expression of GABAR1 increased and the mRNA transcription and protein expression of NMDAR2B decreased compared with control group (P<0.01). Seven days after isoflurane inhalation, the spatial memory ability of group S4 decreased, the mRNA transcription and protein expression of both GABAR1 increased, the mRNA transcription and protein expression of NMDAR2B decreased compared with control group and the other test groups (P<0.01). There was no significant difference between the control group and groups S1, S2, S3. Conclusion Continuous inhalation of isoflurane has great effects on spatial memory ability. And impaired spatial memory by isoflurane inhalation of high concentration with long duration is present in a long time. Thoses are related with the mRNA transcription and protein expressions of GABAR1 and NMDAR2B in cerebral temporal lope.
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