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| 七氟醚单次和多次暴露对新生鼠海马神经元结构的影响 |
| Different effects of a single exposure or multiple exposures to sevoflurane on hippocampal structures in neonatal rats |
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| DOI： |
| 中文关键词: 七氟醚 新生大鼠 神经元 结构 |
| 英文关键词: Sevoflurane Neonatal rat Neuron Structures |
| 基金项目:宁夏自然科学基金资助（NZ15135） |
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| 摘要点击次数: 3018 |
| 全文下载次数: 2017 |
| 中文摘要: |
| 目的 探讨等同时间不同次数七氟醚暴露对新生大鼠海马CA1细胞形态及超微结构的影响。方法 出生7 d的SD雄性大鼠45只,体重14～18 g,随机均分为三组:对照组(C组)、单次七氟醚暴露组(SS组)和多次七氟醚暴露组(TS组)。SS组于出生后第7天吸入1次3%七氟醚6 h;TS组于出生后第7、8、9天每天吸入1次3%七氟醚2 h,累计6 h;C组在相应日龄吸入60%氧气。三组于出生后第14天灌注取脑,采用HE和尼氏染色观察大鼠海马CA1区锥体神经元形态及数量变化;同时,透射电镜下观察该区域神经元超微结构及突触后致密物厚度和活性区长度。结果 HE和尼氏染色显示:与C组比较, SS组和TS组海马CA1细胞排列稀疏,神经元数量明显减少(P＜0.05);与SS组比较,TS组海马CA1神经元数量明显减少(P＜0.05)。电镜结果显示:与C组比较,SS组和TS组海马CA1神经元亚细胞器均有不同程度的受损,突触后致密物厚度明显变薄, 活性区长度明显缩短(P＜0.05);与SS组比较,TS组神经元亚细胞器损伤更明显,突触后致密物厚度更薄, 活性区长度缩短更严重(P＜0.05)。结论 七氟醚单次和多次暴露均会引起新生大鼠海马CA1区锥体神经元数量减少及细胞超微结构的改变,等同时间多次暴露比单次暴露对神经元形态的损伤更严重。 |
| 英文摘要: |
| Objective To investigate the effects of a single exposure or multiple exposures with equivalent total duration of exposure to sevoflurane on the histological morphology and neurons ultrastructure changes in neonatal rats hippocampus CA1. Methods A total of 45 male Sprague-Dawley rats on postnatal day 7, weighing 14-18 g,were randomly divided into three groups (n=15 each): Control group (group C), single exposure to sevoflurane group (group SS), multiple exposures to sevoflurane group (group TS). In group SS, the rats inhaled 3% sevoflurane for 6 h on postnatal day 7. In group TS, the rats inhaled 3% sevoflurane for 2 h on postnatal day 7,8 and 9. In group C, the rats inhaled 60% oxygen on the corresponding day age. Rats were sacrificed and brain were seperated on postnatal day 14. CA1 pyramidal neurons pathological morphology and quantity changes were observed by Hematoxylin-Eosin (HE) and Nissl staining. In the meantime, transmission electron microscopy was used for observing neurons ultrastructure and measuring the thickness of the postsynaptic density and the length of the postsynaptic active area. Results Nissl staining and HE indicated that multiple exposures and a single 6 h exposure to sevoflurane resulted in severer neurons loss and sparse arrangement relative to group C (P＜0.05), Multiple exposures to sevoflurane resulted in greater neurons loss compared with a single 6-h exposure (P＜0.05).Transmission electron microscope indicated that damage of CA1 neuronal subcellular organelle induced by multiple exposures and a single 6 h exposure was severer compared with group C. Both multiple exposures and a single exposure lead to decreased thickness of the postsynaptic density and shorter length of the postsynaptic active area (P＜0.05). Multiple exposures to sevoflurane caused greater damaged than a single exposure (P＜0.05). Conclusion Both a single and multiple exposure to sevoflurane induced CA1 neurons loss and ultrastructure changes in neonatal rats. Compared with a single exposure, multiple exposures to sevoflurane resulted in greater neurons morphology injury. |
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