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| 右美托咪定对免疫低下幼鼠细胞免疫的影响 |
| Effects of dexmedetomidine on cellular immune function of immunosuppression juvenile rats |
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| DOI： |
| 中文关键词: 右美托咪定 脾切除 免疫 T淋巴细胞增殖 T细胞亚群 |
| 英文关键词: Dexmedetomidine Splenectomy Immune T lymphocyte proliferation T lymphocyte subsets |
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| 中文摘要: |
| 目的 评价右美托咪定对脾切除幼鼠外周血T淋巴细胞增殖及T细胞亚群的影响。方法 健康雄性SD大鼠24只,6周龄,体重130～150 g,随机分为四组：生理盐水组（S组）、右美托咪定组（D组）、生理盐水组+脾切除(SN组)和右美托咪定组+脾切除(SD组)，每组6只。S、D组不做外科处理，SN、SD组制备脾切除模型。喂养1周后S组和SN组腹腔注射生理盐水 10 ml/kg,D组和SD组腹腔注射右美托咪定50 μg/kg。给药后2 h,取血样,采用MTT法检测外周血T淋巴细胞增殖能力,采用流式细胞术检测T细胞亚群即CD4+、CD8+细胞含量,计算CD4+/CD8+值。结果 与S组比较,SN组T淋巴细胞增殖能力降低,CD4+、CD8+含量及CD4+/CD8+值降低,D组T淋巴细胞增殖能力明显降低(P＜0.05);与D组比较,SD组T淋巴细胞增殖能力明显降低,CD4+、CD8+含量及CD4+/CD8+值明显降低(P＜0.05);与SN组比较,SD组T淋巴细胞增殖能力明显降低(P＜0.05)。结论 右美托咪定对正常幼鼠和免疫低下幼鼠细胞免疫均有一定抑制作用,且对免疫低下幼鼠的抑制作用更强。 |
| 英文摘要: |
| Objective To explore the effects of dexmedetomidine on peripheral blood T lymphocyte proliferation and T lymphocyte subsets of juvenile rats with splenectomy. Methods Twenty-four healthy male Sprague Dawley rats, weighing 130-150 g, aged six weeks were enrolled in this study. Half of the rats received splenectomy to make an immunosuppressive model,then they were randomly divided into 2 groups (n=6 each): splenectomy+normal saline group (group SN) and splenectomy+dexmedetomidine group (group SD). The another half of the rats without splenectomy were randomly divided into 2 groups: normal saline group (group S) and dexmedetomidine group (group D). After one week of normal feeding, normal saline 10 ml/kg was injected intraperitoneally (ip) in groups S and SN, dexmedetomidine 50 μg/kg was injected ip in groups D and SD respectively. Two hours after the injection, blood samples were collected. MTT was utilized to examine the peripheral blood T lymphocyte proliferative capability. T lymphocyte subsets CD4+, CD8+ were determined by flow cytometry. CD4+/CD8+ was calculated. Results Compared with group S, T lymphocyte proliferative capability, the percentages CD4+, CD8+ and CD4+/CD8+ ratio were significantly decreased in group SN (P＜0.05); T lymphocyte proliferative capability in group D was decreased (P＜0.05), but no significant changes was found in the percentages CD4+, CD8+ and CD4+/CD8+ ratio. Compared with the group D, T lymphocyte proliferative capability, the percentages CD4+, CD8+ and CD4+/CD8+ ratio in group SD were significantly decreased (P＜0.05). Compared with the group SN, T lymphocyte proliferative capability in group SD was significantly decreased (P＜0.05). Conclusion Cellular immune function of juvenile rats with or without splenectomy is suppressed by dexmedetomidine, and the suppressive function is more severe in splenectomy rats than that in normal juvenile rats. |
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