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| 舒芬太尼对小鼠周围神经损伤后脊髓星形胶质细胞活化的影响 |
| Effect of sufentanil on activation of spinal astrocytes in a mice model of single peripheral nerve injury |
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| DOI： |
| 中文关键词: 舒芬太尼 周围神经系统 脊髓 星型胶质细胞 |
| 英文关键词: Sufentanil Peripheral nerve systerm Spinal cord Astrocytes |
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| 中文摘要: |
| 目的 探讨舒芬太尼对小鼠单侧坐骨神经损伤后脊髓星形胶质细胞活化的影响。方法 清洁级健康雄性BALB／c小鼠80只, 6～8周龄,体重 18～22 g。采用随机数字量表法分为四组:舒芬太尼高剂量组(H组)、舒芬太尼中剂量组(M组)、舒芬太尼低剂量组(L组)和模型组(MO组),每组20只。制备单侧坐骨神经离断损伤模型后,H、M、L组分别腹腔注射舒芬太尼10 μg／kg、5 μg／kg、2.5 μg／kg,MO组腹腔注射等容量生理盐水,连续注射3 d,每天1次。于4、8、12周检测小鼠坐骨神经功能指数(SFI)。于2、4、8、12周每组随机处死的5只小鼠,取其损伤同侧L4～L6脊髓节段。光学显微镜下观察病理学结果。采用免疫组化法检测脊髓各时点GFAP的表达。结果 H、M组SFI明显高于L、MO组(P<0.05),L、MO组SFI差异无统计学意义;H、M组脊髓前角神经元形态较好,L、MO组脊髓前角神经元形态较差。与MO组比较,各时点H、M、L组GFAP的表达上调(P<0.05);与L组比较,各时点H、M组GFAP的表达上调(P<0.05)。结论 舒芬太尼促进小鼠周围神经损伤后脊髓星形胶质细胞活化,利于周围神经损伤后的修复。 |
| 英文摘要: |
| Objective To investigate the effects of sufentanil on activation of spinal astrocytes in mice after unilateral sciatic nerve injury. Methods Eighty healthy male BALB／c mice, aged 6-8 weeks, weighing 18-22 g, were randomly divided into 4 groups (n=20 each): sufentanil high dose group (group H), middle dose group (group M), low dose group (group L) and model group (group MO). After model of sciatic nerve injury was established by unilateral sciatic nerve transection, group H, M and L, sufentanil 10 μg／kg, 5 μg／kg, 2.5 μg／kg was injected intraperitoneally once a day continuously for 3 days, while the equal volume of saline was injected in group MO. Five mice in each group were selected, and the sciatic nerve functional index were measured at 4, 8, 12 weeks. The 5 mice were sacrificed from each group and the damage on the same side L4-L6 segments of the spinal cord were removed at 2, 4, 8,12 weeks. The pathological changes were examined under light microscope at 8 week point. The expression of GFAP was determinatied at each time points by immuno-histochemistry. Results Compared with group L and MO, sciatic nerve functional index significantly was increased in groups H and M (P<0.05), and no significantly change was found in group L. Spinal cord neurons had a better morphology in groups H and M than in group L and MO. Compared with group MO, The expression of GFAP were significantly up-regulated in groups H, M and L (P<0.05). Compared with group L, the expression of GFAP were significantly up regulated in groups H and M (P<0.05). Conclusion Sufentanil promotes spinal astrocyte activation after peripheral nerve injury in mice and improves repair after peripheral nerve injury. |
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