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| 帕瑞昔布对肝部分切除术老年大鼠海马炎症反应的影响 |
| Effect of parecoxib on hippocampal inflammation following partial hepatectomy in rats |
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| DOI： |
| 中文关键词: 帕瑞昔布 COX-2 caspase-3 海马 炎症 |
| 英文关键词: Parecoxib COX-2 caspase-3 Hippocampus Inflammation |
| 基金项目:国家自然科学基金青年基金(30901393) |
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| 中文摘要: |
| 目的 研究帕瑞昔布对肝部分切除术老年大鼠海马炎症反应的影响。方法 选择SPF级雄性SD大鼠70只,20月龄,体重500～600 g,随机分为三组：对照组(C组,n=10),手术组(S组,n=30)及帕瑞昔布组(P组,n=30)。采用肝部分切除术建立手术创伤导致海马炎症反应模型。P组大鼠在异氟醚麻醉下行肝部分切除术,并于术前1 h腹腔注射帕瑞昔布10 mg／kg,术后每日腹腔注射帕瑞昔布2 mg／kg直至处死。S组大鼠在异氟醚麻醉下行肝部分切除术,术前1 h腹腔注射与P组等量的生理盐水。C组腹腔注射与以上各组等量的生理盐水。干预后第1、3、7 天处死大鼠,取海马组织,采用实时定量PCR法检测环氧合酶-2(COX-2)mRNA的表达量,Western blot法检测COX-2、半胱氨酸蛋白酶-3(caspase-3)的蛋白表达量,ELISA法检测前列腺素E2(PGE2)的含量。结果 干预后第1、3天S组和P组COX-2 mRNA及蛋白表达、PGE2含量、caspase-3蛋白表达明显高于C组(P<0.05或P＜0.01),但P组明显低于S组(P<0.05或P＜0.01)。干预后第7天三组大鼠COX-2 mRNA及蛋白表达、PGE2含量、caspase-3蛋白表达差异无统计学意义。结论 肝部分切除术可导致海马炎症反应,帕瑞昔布可显著抑制此种炎症反应,其机制可能与下调海马COX-2 mRNA和蛋白表达、PGE2含量和caspase-3蛋白表达有关。 |
| 英文摘要: |
| Objective To investigate the effect of parecoxib on hippocompal inflammation following partial hepatectomy in aged rats. The effects of selective COX-2 inhibitor (parecoxib) on hippocampal inflammation were also evaluated. Methods Seventy male rats, aged 20 months, weighing 500-600 g, were randomly divided into three groups: control (n=10), surgery (n=30), and parecoxib (n=30). Control animals received sterile saline to control for the effects of injection stress. Rats in the surgery group received partial hepatectomy under isoflurane anesthesia and sterile saline injection. Rats in the parecoxib group received surgery and anesthesia similar to surgery group rats, and parecoxib treatment. On postanesthetic day 1, 3 and 7, animals were euthanized to assess the hippocampal COX-2 expression, PGE2 production and caspase-3 expression. Results Surgery significantly increased the expression of COX-2 mRNA and protein expression, PGE2 production and caspase-3 protein expression on day 1 postoperatively (P＜0.01), until day 3 (P<0.05) compared to control group. Parecoxib treatment group significantly suppressed COX-2 mRNA and protein expression, PGE2 production and caspase-3 protein expression on postoperative day 1 and day 3 (P<0.05 or P＜0.01), in comparison with the surgery group. There was no significant difference between control group and other groups of the expression of COX-2 mRNA and protein expression, PGE2 production and caspase-3 protein expression on day 7. Conclusion Partial hepatectomy induces a short-term hippocampal inflammatory response in the rats. Parecoxib suppressed the hippocampal inflammation via the down-regulation of COX-2 mRNA and protein, PGE2 and caspase-3 protein in rats following partial hepatectomy. |
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