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| 脑红蛋白在感染性休克早期大鼠脑组织中的动态变化 |
| Protein content of neuroglobin in rat brain with septic shock |
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| DOI：10.12089/jca.2016.12.014 |
| 中文关键词: 感染性休克 脑红蛋白 细胞凋亡 炎症反应 |
| 英文关键词: Septic shock Neuroglobin Cell apoptosis Inflammatory response |
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| 中文摘要: |
| 目的：探讨感染性休克早期大鼠脑组织中脑红蛋白(neuroglobin,NGB)含量的动态变化在感染性休克中可能发挥的作用。方法健康雄性 SD 大鼠60只,12~15周龄,体重300~350 g,随机分为两组,每组30只。M 组经尾静脉注射脂多糖(LPS)10 mg/kg 制备大鼠感染性休克模型,当 SBP 下降幅度达到基础值的40%时认为感染性休克模型复制成功；C 组给予等量生理盐水。分别在给 LPS 前(T0)、给 LPS 后1 h(T1)、2 h(T2)、4 h(T3)、6 h(T4)采用 ELISA 法检测血清中 TNF-α、IL-6浓度和 NGB 含量,Western blot 法检测脑组织中 NGB、半胱天冬酶-3(caspase-3)的含量。结果与 C 组比较,T1~ T4时 M 组血清中的 TNF-α、IL-6浓度明显增高(P <0.05),脑组织中caspase-3含量明显增加(P <0.05)；T3、T4时血清和脑组织中 NGB 含量明显增高(P <0.05)。结论感染性休克后大鼠脑组织发生了炎症损伤、细胞凋亡病理学改变,脑红蛋白在感染性休克所致的损伤脑组织中含量增加,推测其可能发挥了内源性神经保护功能。 |
| 英文摘要: |
| Objective To observe the expression of neuroglobin in brain of rat with septic shock,and study the protective effect of neuroglobin.Methods Sixty male SD rats were randomly di-vided into two groups,30 rats in each.The rats in group M were injected LPS 10 mg/kg through tail vein to establish septic shock model,the success standard of septic shock was systolic blood pressure dropping to 60% of the basic values;the control group were given equal volume of NS.The levels of rat TNF-α,IL-6 and NGB in serum at the time points of before LPS injection (T0 ),1 h (T1 ),2 h (T2 ),4 h (T3 )and 6 h(T4 )after LPS injection were measured using ELISA kits.NGB and caspase-3 protein expression in brain were determined using Western blotting.Results Compared with group C and T0 ,the TNF-α,IL-6 concentrations in serum and caspase-3 in brain were increased significantly at T1-T4 (P <0.05),NGB protein content in serum and brain at T3 and T4 were increased significantly (P <0.05).Conclusion Cell apoptosis and inflammatory response are discovered in the brain during septic shock.NGB is over-expressed in brain of rats with septic shock,which may play a role of pro-tective function. |
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