|
| 抗坏血酸对糖尿病大鼠肾脏缺血-再灌注后肾小球内皮多糖-蛋白复合物的影响 |
| Effects of ascorbic acid on glomerular endothelial cell protein complex in diabetic rats with renal ischemi-a-reperfusion inj ury |
| |
| DOI： |
| 中文关键词: 抗坏血酸 糖尿病 肾脏 缺血-再灌注损伤 多糖-蛋白复合物 |
| 英文关键词: Ascorbic acid Diabetic nephropathy Renal Ischemia-reperfusion injury Pol-ysaccharide-protein complex |
| 基金项目:江西省南昌市市校合作项目(2012-CYH-SXHZ-YLWS-001) |
|
| 摘要点击次数: 2469 |
| 全文下载次数: 999 |
| 中文摘要: |
| 目的：观察抗坏血酸预处理对糖尿病大鼠肾脏缺血-再灌注后肾小球内皮多糖-蛋白复合物的影响。方法32只 SD 健康清洁大鼠随机均分为四组：A 组为正常血糖大鼠,B、C、D 组首先建立糖尿病模型,A、B 组为假手术组,麻醉后行腹部正中切口,小心分离暴露双侧肾蒂游离肾动脉,30 min 后关闭腹腔；C 组每天腹腔注射生理盐水0.5 ml,15 d 后建立肾脏缺血-再灌注模型；D 组每天腹腔注射抗坏血酸200 mg/kg 0.5 ml,15 d 后建立肾脏缺血-再灌注模型。观察大鼠术前30 min (T1)、游离肾动脉后(T2)、术后30 min(T3)、术后6 h(T4)、术后24 h(T5)的血糖(Glu)、尿素氮(BUN)、肌酐(Cr)、丙二醛(MDA)、超氧化物歧化酶(SOD)的变化,检测术后24 h 半胱天冬酶-3(caspase-3)、细胞色素 C(CytC)和多糖-蛋白复合物的表达情况。结果 C、D 组大鼠在 T3~T5时BUN、Cr、MDA 水平明显高于 A、B 组,SOD 水平明显低于 A、B 组(P <0.05)。与 C 组比较,D 组大鼠 T3~T5时 BUN、Cr、MDA 水平明显降低,SOD 水平明显升高,caspase-3、CytC 表达水平明显降低,肾小球内皮多糖-蛋白复合物的厚度明显增加,肾组织损伤明显改善(P <0.05)。结论抗坏血酸预处理可通过减轻糖尿病大鼠肾缺血-再灌注后肾小球内皮多糖-蛋白复合物的损伤从而减轻糖尿病大鼠肾缺血-再灌注损伤。 |
| 英文摘要: |
| Objective To observe the effect of ascorbic acid preconditioning on the glomerular endothelial polysaccharide protein complex in diabetic rats after renal ischemia and reperfusion. Methods A total of 32 healthy SD rats were randomly divided into 4 groups,group A with normal blood glucose,groups B,C and D diabetic rats,Groups A and B were sham operation groups,after anesthesia,rats were made abdominal median incision and had separation to expose the bilateral renal pedicle and free renal artery carefully,abdominal incision were closed 30 minutes later;rats in group C were injected with normal saline 0.5 ml daily for fifteen days,while rats in group D were injected with ascorbic acid 200 mg/kg (0.5 ml)daily for 1 5 days,both groups C and D then made renal ische-mia reperfusion model;modeling methods:after anesthesia,rats were made abdominal median incision and had separation to expose the bilateral renal pedicle and free renal artery carefully,clipped renal ar-tery with vascular clamp for 30 minutes then released forceps and had reperfusion for 24 hours.To ob-serve the changes of blood glucose,blood urea nitrogen (BUN),creatinine (Scr),malondialdehyde (MDA),superoxide dismutase enzyme (SOD)at 30 minutes before operation (T1 ),after freeing renal artery (T2 ),30 minutes after operation (T3 ),6 hours after operation (T4 )and 24 hours after operation (T5 ),test the expression of caspase-3,cytochrome C (CytC),polysaccharide protein com-plexes after operation for 24 hours.Results In groups C and D,the levels of BUN,SCr and MDA at T3-T5 were significantly higher than those in groups A and B,SOD level was significantly lower than group A and B (P <0.05).The levels of BUN,SCr,MDA at T3-T5 in group D were significantly de-creased,SOD levels were significantly increased,caspase-3 and CytC expression were significantly re-duced and a significant increase in the thickness of the glomerular endothelial polysaccharide protein complex,renal tissue injury significantly improved (P < 0.05 ) when compared with group C. Conclusion Ascorbic acid preconditioning can reduce the injury of renal ischemia reperfusion injury in diabetic rats by reducing the injury of glomerular endothelial protein complex after renal ischemia reperfusion in diabetic rats. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
