|
| 前列地尔预处理对犬失血性休克肺损伤的影响 |
| Protective effects of alprostadil pretreatment on lung injury in dogs with hemorrhagic shock |
| |
| DOI： |
| 中文关键词: 前列地尔 失血性休克 肺损伤 前B细胞集落增强因子 细胞因子 |
| 英文关键词: Alprostadil Hemorrhagic shock Lung injury Pre-B-cell colony-enhancing fac-tor Cell factor |
| 基金项目: |
|
| 摘要点击次数: 2382 |
| 全文下载次数: 1141 |
| 中文摘要: |
| 目的：通过观察前列地尔对犬失血性休克致肺损伤肺组织前 B 细胞集落增强因子(pre-B-cell colony-enhancing factor,PBEF)以及血清细胞因子的影响来探讨前列地尔肺保护的机制。方法实验用杂种犬24只,随机均分为四组：对照组(C 组)、失血性休克组(S 组)、前列地尔30 ng组(P1组)、前列地尔60 ng 组(P2组)。C 组仅行动静脉插管不放血,S 组股动脉放血,MAP 降至(40±5)mm Hg,制备失血性休克急性肺损伤(ALI)模型,间断回输或放血维持此 MAP 90 min,然后股静脉回输全部失血,维持 MAP 休克前水平。P1、P2组分别股静脉持续泵注前列地尔30 ng·kg-1· min-1(P1组)、60 ng·kg-1·min-1(P2组)维持30 min,预处理1 h 后建立失血性休克 ALI 模型。分别于休克前(T0)、休克90 min(T1)、复苏4 h(T4)取动脉血,行动脉血气分析计算氧合指数(OI),取肺组织采用 RT-PCR 技术检测 PBEF mRNA 表达情况；并于 T0、T1、复苏1 h(T2)、复苏2 h(T3)、T4时取2 ml 静脉血通过 ELISA 法检测 TNF-α、IL-1β、IL-10、PBEF 的浓度。结果与 C 组比较, T1、T4时 S、P1和 P2组 OI 明显降低,IL-1β、TNF-a、IL-10、PBEF 浓度和 PBEF mRNA 表达明显升高(P <0.05)；与 S 组比较,T1、T4时 P1和 P2组 OI 明显升高,IL-1β、TNF-α、IL-10、PBEF 浓度和PBEF mRNA 表达明显降低(P <0.05)。结论前列地尔可能通过下调 PBEF 的基因表达,抑制炎症因子 IL-1β、TNF-α、IL-10的释放,从而减轻失血性休克肺损伤。 |
| 英文摘要: |
| Objective To explore the protective mechanism of alprostadil pretreatment in dogs with hemorrhagic shock by observing the variations of pre-B-cell colony-enhancing factor (PBEF)in lung tissue and serum cytokines of the animal.Methods Twenty-four dogs were randomly divided into four groups(n =6),control group (group C),hemorrhagic shock group(group S),group P1 and group P2 received alprostadil 30 ng·kg-1 ·min-1 and 60 ng·kg-1 ·min-1 respectively.In group C, an indwelling catheter were placed in femoral artery and vein of the dog,but no blood was removed from the catheter.Acute lung injury model of hemorrhagic shock was built in group S by removing blood from the femoral artery,and the MAP was maintained at (40±5)mm Hg for 90 min by remo-ving or transfusing blood.MAP was maintained to preoperative level by blood reinfusion through fem-oral vein.Alprostadil was continuously infused at the rate of 30 ng · kg-1 · min-1 and 60 ng·kg-1 ·min-1 in groups P1 and P2 respectively for 30 min before hemorrhagic shock.Samples of arterial blood and lung tissue were obtained before shock (T0 ),90 min after shock (T1 ),and 4 h af-ter recovery (T4 ).Oxygenation index (OI)was calculated in samples of arterial blood.The his-topathological change was observed by light microscope,and the PBEF mRNA level was detected by real-time PCR in lung tissue.Samples of venous blood were taken at T0 ,T1 ,T2 (1 hour after recovery ),T3 (2 hour after recovery ),T4 .The concentration of TNF-α,IL-1β,IL-10 and PBEF were measured by ELISA in these samples.Results Compared with group C,OI was significantly de-creased at T1 ,T4 ,the serum concentrations of IL-1β,TNF-α,IL-10,PBEF and the level of PBEF mRNA expression were significantly increased at T1 ,T4 in groups S,P1 and P2 (P <0.05 );Com-pared with group S,OI was significantly increased at T1 ,T4 ,the serum concentrations of IL-1β, TNF-a,IL-10,PBEF and the level of PBEF mRNA expression were significantly decreased at T1 ,T4 in groups P1 and P2 (P <0.05).Conclusion Alprostadil may reduce lung injury of hemorrhagic shock by decreasing PBEF gene expression and by restraining the release of IL-1β,TNF-αand IL-10. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
