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| 人参皂苷CK对大鼠术后认知功能和线粒体功能的影响 |
| Effects of ginsenoside CK on postoperative cognitive function and mitochondrial function in rats |
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| DOI：10.12089/jca.2025.10.016 |
| 中文关键词: 线粒体自噬 术后认知功能障碍 人参皂苷CK |
| 英文关键词: Mitophagy Postoperative cognitive dysfunction Ginsenoside CK |
| 基金项目:济南市“新高校20条”科研带头人工作室项目（202228124） |
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| 中文摘要: |
目的：探究人参皂苷CK对术后认知功能障碍（POCD）大鼠术后认知功能和线粒体功能的影响，并探讨其机制。
方法：选择SPF级健康雄性SD大鼠27只，28周龄，体重200～220 g。采用随机表法将大鼠分为三组：对照组（C组）、手术模型组（S组）和人参皂苷CK+手术模型组（G组），每组9只。C组不做任何处理；S组行七氧醚剖腹探查术建立POCD模型；G组行七氧醚剖腹探查术，并于术前3 d至术后3 d每天给予人参皂苷CK 20 mg/kg灌胃。于术后3 d行旷场实验记录总移动距离和在中央区域时间，行水迷宫实验记录逃避潜伏期和穿越平台次数，采用RT-qPCR法检测大鼠海马组织线粒体NADH脱氢酶亚基1（MT-ND1）mRNA表达量，采用Western blot法检测海马组织磷酸酶及张力蛋白同源物诱导的蛋白激酶1（PINK1）、帕金蛋白（Parkin）、微管相关蛋白轻链3 Ⅱ（LC 3Ⅱ）、微管相关蛋白轻链3Ⅰ（LC 3Ⅰ）蛋白含量，并计算LC 3 Ⅱ/Ⅰ比值，采用透射电镜检测海马组织超微结构。
结果：与C组比较，S组总移动距离、在中央区域时间明显缩短，穿越平台次数明显减少，逃避潜伏期明显延长，MT-ND1 mRNA表达量明显降低，PINK1、Parkin、LC 3 Ⅱ、LC 3Ⅰ蛋白含量明显升高，LC 3 Ⅱ/Ⅰ比值明显降低（P<0.05）。与S组比较，G组总移动距离、在中央区域时间明显延长，穿越平台次数明显增多，逃避潜伏期明显缩短，MT-ND1 mRNA表达量明显升高，PINK1、Parkin、LC 3 Ⅱ、LC 3Ⅰ蛋白含量明显降低，LC 3 Ⅱ/Ⅰ比值明显升高（P<0.05）。电镜下S组细胞损伤和线粒体损伤严重，G组海马组织细胞损伤轻，线粒体结构较为完整，自噬体表达量稍多，自噬溶酶体内容物基本降解。
结论：人参皂苷CK可通过增强自噬清除能力、恢复线粒体稳态，改善POCD大鼠术后认知功能和线粒体功能。 |
| 英文摘要: |
Objective: To investigate the effects of ginsenoside CK on postoperative cognitive function and mitochondrial function in rats with postoperative cognitive dysfunction (POCD), and explore its mechanism.
Methods: Twenty-seven SPF-grade healthy male SD rats, 28 weeks old, weighing 200-220 g, were randomly divided into three groups using a random number table: control group (group C), surgery and model group (group S), and ginsenoside CK + surgery and model group (group G), 9 rats in each group. Group C received no intervention, group S underwent exploratory laparotomy using sevoflurane to establish POCD model, and group G underwent exploratory laparotomy using sevoflurane and was gavaged with ginsenoside CK 20 mg/kg daily from 3 days before surgery to 3 days after surgery. The cognitive function was evaluated via the open-field test and morris water maze test 3 days after surgery, total moving distance, time in the central area, escape latency and platform crossing times were recorded. Mitochondrially encoded nadh:ubiquinone oxidoreductase core subunit 1 (MT-ND1) mRNA was detected by RT-qPCR. PTEN induced putative kinase 1 (PINK1), Parkinson disease protein 2 (Parkin), microtubule-associated protein 1 light chain 3-Ⅱ (LC 3Ⅱ), LC3Ⅱ, LC3Ⅰ were quantified by Western blot, and LC3Ⅱ/Ⅰ ratio was calculated. Ultrastructure of hippocampal tissue was observed via transmission electron microscopy.
Results: Compared with group C, the total moving distance and platform crossing times were significantly decreased, the central area time was significantly shortened, the escape latency was significantly prolonged, the expression of MT-ND1 mRNA, the content of PINK1, Parkin, LC3Ⅱ, and LC3Ⅰ protein in group S were significantly increased, while the LC3Ⅱ/Ⅰ ratio was significantly reduced (P < 0.05). Compared with group S, the total moving distance and platform crossing times were significantly increased, the central area time was significantly prolonged, the escape latency was significantly shortened, the expression of MT-ND1 mRNA, the content of PINK1, Parkin, LC3Ⅱ, and LC3Ⅰ protein in group G were significantly reduced, while the LC3Ⅱ/Ⅰ ratio was significantly increased (P < 0.05). Electron microscopy showed severe cellular/mitochondrial damage in group S. Group G had milder damage, intact mitochondrial structure, increased autophagosome expression, and degraded autolysosome contents.
Conclusion: Ginsenoside CK improves postoperative cognitive function and mitochondrial function in POCD rats by enhancing autophagic clearance and restoring mitochondrial homeostasis. |
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