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| 神经刺激器引导坐骨神经联合股神经阻滞小鼠动物模型的制备 |
| Establishment of a sciatic-femoral nerve combined block model in mice guided by a nerve stimulator |
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| DOI：10.12089/jca.2025.09.012 |
| 中文关键词: 小鼠 坐骨神经联合股神经阻滞 神经刺激器 解剖标志定位 布比卡因脂质体 |
| 英文关键词: Mice Sciatic-femoral nerve block Nerve stimulator Anatomical landmark location Liposomal bupivacaine |
| 基金项目:南京市卫生科技发展专项资金项目计划（YKK21128）；中国红十字基金会医学赋能公益专项基金（CRCF-YXFN-202302144） |
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| 中文摘要: |
目的：评估神经刺激器引导下1.3%布比卡因脂质体在小鼠坐骨神经联合股神经阻滞（SFNB）动物模型的制备效果。
方法：选择雄性 C57BL/6J 小鼠49只，18月龄，体重28～32 g。实验一：选择34只小鼠进行神经阻滞效果评估，采用随机数字表法分为两组：神经刺激器引导组（S组）和解剖标志定位组（C组），每组17只。S组采用神经刺激器引导行SFNB，C组采用解剖标志定位行SFNB。采用机械刺激缩足反应阈值（PWMT） 和4级评分法，分别于阻滞前1 d和阻滞后2、6、24、36、48 h评估两组阻滞成功的小鼠感觉和运动阻滞程度。实验二：选择剩余15只小鼠进行神经染色研究，以上述方法于每只小鼠两侧分别采用神经刺激器引导（S1组）或解剖标志定位（C1组）行坐骨神经和股神经亚甲蓝染色，评估阻滞后小鼠神经染色分级和神经Ⅲ级染色长度。
结果：实验一：与C组比较，S组阻滞成功率明显升高［15只（88.2%） vs. 9只（52.9%），P＜0.05］，阻滞后6、24 h PWMT明显升高（P＜0.05）。与阻滞前1 d比较，阻滞后2、6、24、36 h S组PMWT明显升高，阻滞后2 h C组PMWT明显升高（P＜0.05）。实验二：与C1组比较，S1组坐骨神经和股神经染色分级明显升高，Ⅲ级染色长度明显延长（P＜0.05）。
结论：与解剖标志定位比较，神经刺激器引导下1.3%布比卡因脂质体SFNB的小鼠模型具有阻滞成功率高、感觉阻滞持续时间长、神经染色均匀的优势，是一种较为理想的坐骨神经联合股神经阻滞的实验动物模型。 |
| 英文摘要: |
Objective: To evaluate the efficacy of 1.3% liposomal bupivacaine in sciatic-femoral nerve block (SFNB) using nerve stimulator-guided technique in a mouse model.
Methods: Forty-nine male C57BL/6J mice, aged 18 months, weighing 28-32 g, were used in this experiment. In experiment 1, 34 mice were selected to evaluate the effect of nerve block. They were divided into two groups by random number table method: the nerve stimulator-guided group (group S) and the anatomical landmark location group (group C), 17 mice in each group. Group S received SFNB under the guidance of nerve stimulators, while group C received SFNB using the anatomical landmark localization method. The mechanical paw withdrawal threshold (PWMT) and a 4-point scale were employed to assess the degree of sensory and motor blockade in successfully blocked mice 1 day before block and 2, 6, 24, 36, and 48 hours after block. In experiment 2, the remaining 15 mice were selected for the nerve staining study. The sciatic nerves and femoral nerves methylene blue staining were performed on both sides of each mouse respectively under the guidance of a nerve stimulator (described as group S1) or at the location of anatomical landmarks (described as group C1), and the nerve staining grade and the length of grade III nerve staining of the mice after block were evaluated.
Results: Experiment 1: compared with group C, the success rate of block in group S was significantly increased [15 mice (88.2%) vs 9 mice (52.9%), P < 0.05], and the PWMT 6 and 24 hours after block was significantly increased in group S (P < 0.05). Compared with 1 day before block, the PMWT in group S were significantly increased 2, 6, 24, and 36 hours after block, and the PMWT in group C were significantly increased 2 hours after block (P < 0.05). Experiment 2: compared with group C1, the staining grades of the sciatic nerve and femoral nerve were significantly increased, and the length of grade III staining was significantly prolonged in group S1 (P < 0.05).
Conclusion: Compared to anatomical landmark-based positioning, the mouse model of sciatic nerve combined with femoral nerve block (SFNB) using a nerve stimulator-guided 1.3% liposomal bupivacaine demonstrates advantages such as a higher success rate of blockade, prolonged sensory blockade duration, and uniform nerve staining, making it a relatively ideal experimental animal model for this procedure. |
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