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| 人参皂苷CK对大鼠术后认知功能的影响 |
| Effect of Ginsenoside CK on postoperative cognitive function in rats |
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| DOI：10.12089/jca.2025.07.012 |
| 中文关键词: 术后认知功能障碍 人参皂苷CK 氧化应激 炎症反应 |
| 英文关键词: Postoperative cognitive dysfunction Ginsenoside CK Oxidative stress Inflammation |
| 基金项目:济南市“新高校20条”科研带头人工作室项目（202228124） |
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| 中文摘要: |
目的：探讨人参皂苷CK对大鼠术后认知功能的影响及可能机制。
方法：选择SPF级健康雄性SD大鼠30只，28周龄，体重200～220 g。采用随机表法将大鼠分为三组：对照组（C组）、手术组（S组）和人参皂苷CK+手术组（G组），每组10只。C组不做任何处理；S组行剖腹探查术；G组行剖腹探查术，并于术前3 d至术后3 d，每日予人参皂苷CK 20 mg/kg灌胃。于术后1、3 d行旷场实验记录总移动距离和在中央区域停留时间，行水迷宫实验记录逃避潜伏期和穿越平台次数，采用ELISA法检测海马组织活性氧（ROS）、丙二醛（MDA）、白细胞介素（IL）-18和IL-1β浓度，采用Western blot法检测海马组织NOD样受体家族含pyrin结构域蛋白3（NLRP3）、凋亡相关斑点样蛋白（ASC）、半胱天冬酶-1前体（pro-Caspase-1）和半胱天冬酶-1（Caspase-1）蛋白含量。
结果：与C组比较，术后1、3 d S组总移动距离、在中央区域停留时间明显缩短，逃避潜伏期明显延长，穿越平台次数明显减少，ROS、MDA、IL-1、IL-1β浓度、NLRP3、ASC、pro-Caspase-1、Caspase-1蛋白含量均明显升高（P<0.05）。与S组比较，术后1、3 d G组总移动距离、在中央区域停留时间明显延长，逃避潜伏期明显缩短，穿越平台次数明显增多，ROS、MDA、IL-1、IL-1β浓度、NLRP3、ASC、pro-Caspase-1、Caspase-1蛋白含量均明显降低（P<0.05）。
结论：人参皂苷CK能够通过抑制NLRP3炎性小体活化，减轻大鼠海马组织氧化应激损伤和炎症反应，改善大鼠术后认知功能。 |
| 英文摘要: |
Objective: To investigate the effect and potential mechanism of Ginsenoside CK on postoperative cognitive function in rats.
Methods: Thirty SPF-grade healthy male Sprague-Dawley rats, 28 weeks old, weighing 200-220 g, were randomly divided into three groups using a random number table: control group (group C), surgery group (group S), and ginsenoside CK + surgery group (group G), 10 rats in each group. Group C received no intervention. Group S underwent sevoflurane anesthesia and exploratory laparotomy. Group G received sevoflurane anesthesia, exploratory laparotomy, and Ginsenoside CK 20 mg/kg via gavage from 3 days preoperatively to 3 days postoperatively. Total movement distance and time in the central area in the open field test, escape latency and number of platform crossings in the morris water maze test, the concentrations of reactive oxygen species (ROS), malondialdehyde (MDA), interleukin (IL)-18, and IL-1β in hippocampal tissues by ELISA test, and the contents of NOD-like receptor thermal protein domain associated protein 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD (ASC), pro-Caspase-1, and Caspase-1 in hippocampal tissues by Western blot were recorded 1 day and 3 days after surgery.
Results: Compared with group C, the total movement distance and time in the central area were significantly shortened, escape latency were significantly prolonged, number of platform crossings were significantly decreased, the concentrations of ROS, MDA, IL-18, and IL-1β were significantly increased, the contents of NLRP3, ASC, pro-Caspase-1, and Caspase-1 were significantly increased in group S 1 day and 3 days after surgery (P < 0.05). Compared with group S, the total movement distance and time in the central area were significantly prolonged, escape latency were significantly shortened, number of platform crossings were significantly increased, the concentrations of ROS, MDA, IL-18, and IL-1β were significantly decreased, the contents of NLRP3, ASC, pro-Caspase-1, and Caspase-1 were significantly decreased in group G 1 day and 3 days after surgery (P < 0.05).
Conclusion: Ginsenoside CK can reduce oxidative stress and neuroinflammation in the hippocampus by inhibiting NLRP3 inflammasome activation, thereby ameliorating postoperative cognitive function. |
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